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首页> 外文期刊>Molecular and Cellular Biology >Interplay between Chromatin and trans-Acting Factors on the IME2 Promoter upon Induction of the Gene at the Onset of Meiosis
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Interplay between Chromatin and trans-Acting Factors on the IME2 Promoter upon Induction of the Gene at the Onset of Meiosis

机译：减数分裂发作时基因诱导后，染色质与IME2启动子上反式作用因子之间的相互作用

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摘要

The IME2 gene is one of the key regulators of the initiation of meiosis in budding yeast. This gene is repressed during mitosis through the repressive chromatin structure at the promoter, which is maintained by the Rpd3-Sin3 histone deacetylase (HDAC) complex. IME2 expression in meiosis requires Gcn5/histone acetyltransferase, the transcriptional activator Ime1, and the chromatin remodeler RSC; however, the molecular basis of IME2 activation had not been previously defined. We found that, during mitotic growth, a nucleosome masked the TATA element of IME2, and this positioning depended on HDAC. This chromatin structure was remodeled at meiosis by RSC that was recruited to TATA by Ime1. Stable tethering of Ime1 to the promoter required the presence of Gcn5. Interestingly, Ime1 binding to the promoter was kept at low levels during the very early stages in meiosis, even when the levels of Ime1 and histone H3 acetylation at the promoter were at their highest, making a 4- to 6-h delay of the IME2 expression from that of IME1. HDAC was continuously present at the promoter regardless of the transcriptional condition of IME2, and deletion of RPD3 allowed the IME2 expression shortly after the expression of IME1, suggesting that HDAC plays a role in regulating the timing of IME2 expression.
机译： IME2 基因是发芽酵母中减数分裂启动的关键调控因子之一。该基因在有丝分裂期间通过启动子处的抑制性染色质结构被抑制，该结构由Rpd3-Sin3组蛋白脱乙酰基酶（HDAC）复合物维持。减数分裂中的 IME2 表达需要Gcn5 /组蛋白乙酰转移酶，转录激活因子Ime1和染色质重塑剂RSC。但是， IME2 激活的分子基础尚未预先定义。我们发现，在有丝分裂生长期间，核小体掩盖了 IME2 的TATA元件，而这种定位取决于HDAC。该染色质结构在减数分裂中被RSC重塑，RSC被Ime1募集到TATA。 Ime1与启动子的稳定连接需要Gcn5的存在。有趣的是，即使在启动子处的Ime1和组蛋白H3乙酰化水平最高时，减数分裂的早期阶段，Ime1与启动子的结合仍保持在较低水平，从而使 IME2 表达式与 IME1 表达式相同。 HDAC持续存在于启动子上，而与 IME2 的转录条件无关，并且 RPD 3的缺失使 IME2 表达在表达后不久出现。 IME1 ，表明HDAC在调节 IME2 表达时间方面发挥了作用。

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