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首页> 外文期刊>Molecular and Cellular Biology >Stb1 Collaborates with Other Regulators To Modulate the G1-Specific Transcriptional Circuit
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Stb1 Collaborates with Other Regulators To Modulate the G1-Specific Transcriptional Circuit

机译：Stb1与其他调节器协作以调节G1特定的转录电路

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G1-specific transcription in the budding yeast Saccharomyces cerevisiae depends upon SBF and MBF. Whereas inactivation of SBF-regulated genes during the G1/S transition depends upon mitotic B-type cyclins, inactivation of MBF has been reported to involve multiple regulators, Nrm1 and Stb1. Nrm1 is a transcriptional corepressor that inactivates MBF-regulated transcription via negative feedback as cells exit G1 phase. Cln/cyclin-dependent kinase (CDK)-dependent inactivation of Stb1, identified via its interaction with the histone deacetylase (HDAC) component Sin3, has also been reported to inactivate MBF-regulated transcription. This report shows that Stb1 is a stable component of both SBF and MBF that binds G1-specific promoters via Swi6 during G1 phase. It is important for the growth of cells in which SBF or MBF is inactive. Although dissociation of Stb1 from promoters as cells exit G1 correlates with Stb1 phosphorylation, phosphorylation is only partially dependent upon Cln1/2 and is not involved in transcription inactivation. Inactivation depends upon Nrm1 and Clb/CDK activity. Stb1 inactivation dampens maximal transcriptional induction during late G1 phase and also derepresses gene expression in G1-phase cells prior to Cln3-dependent transcriptional activation. The repression during G1 also depends upon Sin3. We speculate that the interaction between Stb1 and Sin3 regulates the Sin3/HDAC complex at G1-specific promoters.
机译：芽生酵母中的G 1 特异转录取决于SBF和MBF。尽管在G 1 / S过渡过程中SBF调控基因的失活取决于有丝分裂B型细胞周期蛋白，但据报道MBF失活涉及多个调节子Nrm1和Stb1。 Nrm1是一种转录共抑制因子，当细胞退出G 1 期时，它会通过负反馈使MBF调控的转录失活。还已经报道了通过Stb1与组蛋白脱乙酰基酶（HDAC）组分Sin3相互作用鉴定的Cln /细胞周期蛋白依赖性激酶（CDK）依赖性失活可以灭活MBF调节的转录。该报告表明，Stb1是SBF和MBF的稳定成分，在G 1 阶段通过Swi6与G 1 特异性启动子结合。这对于SBF或MBF失活的细胞的生长很重要。尽管随着细胞退出G 1 ，Stb1与启动子的解离与Stb1磷酸化有关，但磷酸化仅部分依赖于Cln1 / 2，并且不参与转录失活。灭活取决于Nrm1和Clb / CDK活性。 Stb1失活抑制了G 1 晚期的最大转录诱导，并抑制了Cln3依赖性转录激活之前G 1 期细胞的基因表达。 G 1 期间的抑制也取决于Sin3。我们推测Stb1和Sin3之间的相互作用调节G 1 特异性启动子上的Sin3 / HDAC复合物。

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