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首页> 外文期刊>Molecular and Cellular Biology >Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity
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Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity

机译：CTCF C末端的特定位点与黏附素复合物的SA2亚基相互作用，并且是黏附素依赖性绝缘活性所必需的

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Recent studies have shown that the protein CTCF, which plays an important role in insulation and in large-scale organization of chromatin within the eukaryotic nucleus, depends for both activities on recruitment of the cohesin complex. We show here that the interaction of CTCF with the cohesin complex involves direct contacts between the cohesin subunit SA2 and specific regions of the C-terminal tail of CTCF. All other cohesin components are recruited through their interaction with SA2. Expression in vivo of CTCF mutants lacking the C-terminal domain, or with mutations at sites within it required for SA2 binding, disrupts the normal expression profile of the imprinted genes IGF2-H19 and also results in a loss of insulation activity. Taken together, our results demonstrate that specific sites on the C terminus of CTCF are essential for cohesin binding and insulator function. The only direct interaction between CTCF and cohesin involves contact with SA2, which is external to the cohesin ring. This suggests that in recruiting cohesin to CTCF, SA2 could bind first and the ring could assemble subsequently.
机译：最近的研究表明，蛋白质CTCF在真核中的绝缘和染色质的大规模组织中起着重要作用，而这两种活动都依赖于黏附素复合物的募集。我们在这里显示，CTCF与黏附素复合物的相互作用涉及黏附素亚基SA2与CTCF C末端尾部的特定区域之间的直接接触。所有其他黏附素成分均通过与SA2的相互作用而募集。缺少C端结构域或SA2结合所需的位点上有突变的CTCF突变体的体内表达 破坏了印迹基因 IGF2-H19 并且还会导致绝缘活性下降。两者合计，我们的结果表明，CTCF C末端的特定位点对于粘附素结合和绝缘子功能至关重要。 CTCF和黏附素之间唯一的直接相互作用是与黏附素环外部的SA2接触。这表明在将粘着蛋白募集到CTCF时，SA2可以先结合，然后再装配环。

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