A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs

Azariyas A. Challa; Branko Stefanovic

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A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs

机译：波形蛋白细丝的新型作用：胶原mRNA的结合和稳定。

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The stem-loop in the 5′ untranslated region (UTR) of collagen α1(I) and α2(I) mRNAs (5′SL) is the key element regulating their stability and translation. Stabilization of collagen mRNAs is the predominant mechanism for high collagen expression in fibrosis. LARP6 binds the 5′SL of α1(I) and α2(I) mRNAs with high affinity. Here, we report that vimentin filaments associate with collagen mRNAs in a 5′SL- and LARP6-dependent manner and stabilize collagen mRNAs. LARP6 interacts with vimentin filaments through its La domain and colocalizes with the filaments in vivo. Knockdown of LARP6 by small interfering RNA (siRNA) or mutation of the 5′SL abrogates the interaction of collagen mRNAs with vimentin filaments. Vimentin knockout fibroblasts produce reduced amounts of type I collagen due to decreased stability of collagen α1(I) and α2(I) mRNAs. Disruption of vimentin filaments using a drug or by expression of dominant-negative desmin reduces type I collagen expression, primarily due to decreased stability of collagen mRNAs. RNA fluorescence in situ hybridization (FISH) experiments show that collagen α1(I) and α2(I) mRNAs are associated with vimentin filaments in vivo. Thus, vimentin filaments may play a role in the development of tissue fibrosis by stabilizing collagen mRNAs. This finding will serve as a rationale for targeting vimentin in the development of novel antifibrotic therapies.

机译：胶原α1（I）和α2（I）mRNA（5'SL）的5'非翻译区（UTR）中的茎环是调节其稳定性和翻译的关键元素。胶原mRNA的稳定化是纤维化中胶原高表达的主要机制。 LARP6以高亲和力结合α1（I）和α2（I）mRNA的5'SL。在这里，我们报告波形蛋白细丝以5'SL和LARP6依赖的方式与胶原mRNA关联并稳定胶原mRNA。 LARP6通过其La结构域与波形蛋白丝相互作用，并与体内丝共定位。 LARP6的小干扰RNA（siRNA）的敲除或5'SL的突变消除了胶原mRNA与波形蛋白丝的相互作用。波形蛋白敲除成纤维细胞产生的I型胶原蛋白量减少，原因是胶原蛋白α1（I）和α2（I）mRNA的稳定性降低。使用药物或通过显性阴性结蛋白的表达破坏波形蛋白细丝会降低I型胶原蛋白的表达，这主要是由于胶原蛋白mRNA的稳定性降低。 RNA荧光原位杂交（FISH）实验表明，胶原α1（I）和α2（I）mRNA与体内波形蛋白细丝相关。因此，波形蛋白细丝可通过稳定胶原mRNA来在组织纤维化发展中发挥作用。该发现将作为在新型抗纤维化疗法发展中靶向波形蛋白的理由。 展开▼

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《Molecular and Cellular Biology》 |2011年第18期|共17页

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Azariyas A. Challa; Branko Stefanovic;
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中图分类细胞生物学;

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1. Osteoblastic alkaline phosphatase mRNA is stabilized by binding to vimentin intermediary filaments [J] . Schmidt Yvonne, Biniossek Martin, Stark G. Bjoern, Biological chemistry . 2015,第3期

机译：成骨细胞碱性磷酸酶mRNA通过与波形蛋白中间丝结合而稳定

2. A novel taxol-induced vimentin phosphorylation and stabilization revealed by studies on stable microtubules and vimentin intermediate filaments. [J] . Vilalta PM, Zhang L, Hamm-Alvarez SF Journal of Cell Science . 1998,第Pta13期

机译：通过对稳定的微管和波形蛋白中间丝的研究揭示了一种新型的紫杉醇诱导波形蛋白的磷酸化和稳定作用。

3. A novel taxol-induced vimentin phosphorylation and stabilization revealed by studies on stable microtubules and vimentin intermediate filaments [J] . Vilalta PM., Hamm-Alvarez SF., Zhang LP. Journal of Cell Science . 1998,第Pta13期

机译：通过稳定的微管和波形蛋白中间丝的研究揭示了一种新型的紫杉醇诱导波形蛋白的磷酸化和稳定化。

4. Structural Role of Tyrosine Residue in the Third Repeat of Microtubule Binding Domain for Filament Formation of Tau Protein [C] . Katsuhiko Minoura, Kengo Takeuchi, Chisato Nishiura Japanese peptide symposium . 2010

机译：酪氨酸残基在微管结合结构域的三角形蛋白形成酪氨酸残基的结构作用

5. Understanding the functions of vimentin filaments in collagen expression and targeting vimentin filaments for the treatment of fibrosis. [D] . Challa, Azariyas. 2011

机译：了解波形蛋白丝在胶原蛋白表达中的功能，并针对波形蛋白丝治疗纤维化。

6. A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs [O] . Azariyas A. Challa, Branko Stefanovic 2011

机译：波形蛋白细丝的新型作用：胶原mRNA的结合和稳定。

7. A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs ▿ [O] . Challa, Azariyas A., Stefanovic, Branko 2011

机译：波形蛋白细丝的新型作用：胶原mRNA的结合和稳定。 ▿

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2. 肝脏细胞胶原合成及其I型胶原mRNA的稳定性 [J] . 刘树人 ,顾鹤定 . 胃肠病学和肝病学杂志 . 1998,第004期

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4. PDGF与肝素对动脉SMCⅠ、Ⅲ型前胶原mRNA表达及胶原蛋白合成的作用 [J] . 杨方 ,赵培真 ,佘铭鹏 . 中国医学科学院学报 . 1999,第001期

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7. 利用蛋白质组学发现机械力传导过程中的反式作用因子和细丝蛋白A结合伴侣的研究 [A] . Jiale WANG(王家乐) . 2019

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2. 特异性结合分离的衍生自波形蛋白的肽的抗体或该肽的结合片段 [P] . 中国专利： CN108026167A . 2018-05-11

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机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法

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机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途

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机译：内皮素-1 mRNA表达抑制剂，基质金属蛋白酶9 mRNA表达抑制剂，胶原蛋白生产促进剂，原纤蛋白生产促进剂，纤丝蛋白生产促进剂和水合蛋白3 mRNA表达促进剂

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A Novel Role of Vimentin Filaments: Binding and Stabilization of Collagen mRNAs

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