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首页> 外文期刊>Molecular and Cellular Biology >Mitotic Exit Regulation through Distinct Domains within the Protein Kinase Cdc15
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Mitotic Exit Regulation through Distinct Domains within the Protein Kinase Cdc15

机译：通过蛋白激酶Cdc15中的不同域的有丝分裂退出调节。

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The mitotic exit network (MEN), a Ras-like signaling cascade, promotes the release of the protein phosphatase Cdc14 from the nucleolus and is essential for cells to exit from mitosis in Saccharomyces cerevisiae. We have characterized the functional domains of one of the MEN components, the protein kinase Cdc15, and investigated the role of these domains in mitotic exit. We show that a region adjacent to Cdc15's kinase domain is required for self-association and for binding to spindle pole bodies and that this domain is essential for CDC15 function. Furthermore, we find that overexpression of CDC15 lacking the C-terminal 224 amino acids results in hyperactivation of MEN and premature release of Cdc14 from the nucleolus, suggesting that this domain within Cdc15 functions to inhibit MEN signaling. Our findings indicate that multiple modes of MEN regulation occur through the protein kinase Cdc15.
机译：有丝分裂出口网络（MEN）是一种类似Ras的信号传导级联，可促进蛋白磷酸酶Cdc14从核仁中释放出来，对于细胞从酿酒酵母（Saccharomyces cerevisiae）中的有丝分裂中退出至关重要。我们已经表征了MEN组件之一的功能域，即蛋白激酶Cdc15，并研究了这些域在有丝分裂出口中的作用。我们表明，与Cdc15激酶结构域相邻的区域对于自我缔合和与纺锤极体的结合是必需的，并且该结构域对于 CDC15 功能至关重要。此外，我们发现缺少C末端224个氨基酸的 CDC15 的过表达导致MEN的过度活化和Cdc14从核仁的过早释放，表明Cdc15内的该结构域起抑制MEN信号传导的作用。我们的发现表明，MEN调节的多种模式通过蛋白激酶Cdc15发生。

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