U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production

Vera Atzorn; Paola Fragapane; Tamás Kiss

首页> 外文期刊>Molecular and Cellular Biology >U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production

【24h】

U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production

机译：U17 / snR30是一种普遍存在的snoRNA，具有两个18S rRNA生产所必需的保守序列基序。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Saccharomyces cerevisiae snR30 is an essential box H/ACA small nucleolar RNA (snoRNA) required for the processing of 18S rRNA. Here, we show that the previously characterized human, reptilian, amphibian, and fish U17 snoRNAs represent the vertebrate homologues of yeast snR30. We also demonstrate that U17/snR30 is present in the fission yeast Schizosaccharomyces pombe and the unicellular ciliated protozoan Tetrahymena thermophila. Evolutionary comparison revealed that the 3′-terminal hairpins of U17/snR30 snoRNAs contain two highly conserved sequence motifs, the m1 (AUAUUCCUA) and m2 (AAACCAU) elements. Mutation analysis of yeast snR30 demonstrated that the m1 and m2 elements are essential for early cleavages of the 35S pre-rRNA and, consequently, for the production of mature 18S rRNA. The m1 and m2 motifs occupy the opposite strands of an internal loop structure, and they are located invariantly 7 nucleotides upstream from the ACA box of U17/snR30 snoRNAs. U17/snR30 is the first identified box H/ACA snoRNA that possesses an evolutionarily conserved role in the nucleolytic processing of eukaryotic pre-rRNA.

机译：酿酒酵母snR30是加工18S rRNA所必需的H / ACA小盒核仁小RNA（snoRNA）。在这里，我们显示以前表征的人，爬行动物，两栖动物和鱼类U17 snoRNAs代表了酵母snR30的脊椎动物同源物。我们还证明了U17 / snR30存在于裂变酵母 Schizosaccharomyces pombe 和单细胞纤毛原生动物 Teyrhymena thermophila 中。进化比较显示，U17 / snR30 snoRNA的3'末端发夹含有两个高度保守的序列基序，即m1（AUAUUCCUA）和m2（AAACCAU）元素。酵母snR30的突变分析表明，m1和m2元素对于35S pre-rRNA的早期切割至关重要，因此对于成熟的18S rRNA的生产也至关重要。 m1和m2基序占据内部环结构的相反链，并且它们始终位于U17 / snR30 snoRNA的ACA框上游7个核苷酸处。 U17 / snR30是第一个鉴定的H / ACA盒snoRNA，在真核前rRNA的核酸裂解过程中具有进化上保守的作用。 展开▼

著录项

来源
《Molecular and Cellular Biology》 |2004年第4期|共10页

作者
Vera Atzorn; Paola Fragapane; Tamás Kiss;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类细胞生物学;

关键词

相似文献

外文文献

专利

1. Evolutionarily conserved structural features in the ITS2 of mammalian pre-rRNAs and potential interactions with the snoRNA U8 detected by comparative analysis of new mouse sequences. [J] . Michot B, Joseph N, Mazan S, Nucleic Acids Research . 1999,第11期

机译：哺乳动物pre-rRNA的ITS2中的进化保守结构特征以及与snoRNA U8的潜在相互作用（通过对新小鼠序列的比较分析进行检测）。

2. Evolutionarily conserved structural features in the ITS2 of mammalian pre-rRNAs and potential interactions with the snoRNA U8 detected by comparative analysis of new mouse sequences [J] . B. Michot, J. P. Bachellerie, N. Joseph, Nucleic acids research . 1999,第11期

机译：通过对新小鼠序列的比较分析检测到哺乳动物pre-rRNA的ITS2中的进化保守结构特征以及与snoRNA U8的潜在相互作用

3. The 3′-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequence include an invariant region complementary to eukaryotic 5S rRNA [J] . Ahmed A. Azad, Nicholas J. Deacon Nucleic acids research . 1980,第19期

机译：通过直接化学测序方法确定了五个真核18S rRNA的3'-末端一级结构。高度保守的序列包括与真核5S rRNA互补的恒定区

4. Molecular Phylogeny of Protura Inferred from 18S rRNA Gene Sequences [C] . Yun Bu, Yan Gao, Yunxia Luan, 第二届生物计算：理论及应用国际会议（The Second International Confere . 2007

机译：从18S rRNA基因序列推断Protura的分子系统发育。

5. Qualitative assessments and computational techniques for the studies of microbial diversity based on terminal restriction fragment length polymorphism (T-RFLP) of 16S and 18S rRNA gene sequences [D] . Shyu, Conrad. 2006

机译：基于16S和18S rRNA基因序列的末端限制性片段长度多态性（T-RFLP）的微生物多样性研究的定性评估和计算技术

6. U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production [O] . Vera Atzorn, Paola Fragapane, Tamás Kiss 2004

机译：U17 / snR30是一种普遍存在的snoRNA具有两个18S rRNA生产所必需的保守序列基序。

7. U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production [O] . Atzorn, Vera, Fragapane, Paola, Kiss, Tamás 2004

机译：U17 / snR30是一种普遍存在的snoRNA，具有两个18S rRNA生产所必需的保守序列基序。

8. Comparison of the Nucleotide Sequence of Soybean 18S rRNA with the Sequences of Other Small-Subunit rRNAs [R] . Eckenrode, V. K., Arnold, J., Meagher, R. B. 1985

机译：大豆18s rRNa核苷酸序列与其他小亚基rRNa序列的比较

1. 一种18mm、20mm轧带肋钢筋2线切分通用孔型生产方法 [P] . 中国专利： CN113828634A . 2021-12-24

2. Link Protein interleukin - 18 (il-18bp), DNA sequences coding for the IL - 18 BP sequences of cDNA, a vehicle of expression is replicable, host Cell, a process for its production and Insulation, Antibodies, Pharmaceutical compositions and the use of said compositions for [P] . 外国专利： AR062867A2 . 2008-12-10

机译：连接蛋白白介素-18（il-18bp），编码IL-18 BP cDNA的DNA序列，可复制的表达载体，宿主细胞，其生产和绝缘过程，抗体，药物组合物和用途所述组合物用于

3. Use of IL-18 inhibitor, use of an expressing vector which contains a sequence encoding IL-18 inhibitor, use of the vector for induction and/or amplifying endogenic production of IL-18 inhibitor in a cell and use a cell with production of IL-18 inhibitor [P] . 外国专利： SK287761B6 . 2011-09-05

机译：IL-18抑制剂的用途，含有编码IL-18抑制剂序列的表达载体的用途，该载体在细胞中诱导和/或扩增IL-18抑制剂的内源性生产的用途以及使用产生IL的细胞-18抑制剂

4. Use of IL-18 inhibitor, use of an expressing vector which contains a sequence encoding IL-18 inhibitor, use of the vector for induction and/or amplifying endogenic production of IL-18 inhibitor in a cell and use a cell with production of IL-18 inhibitor [P] . 外国专利： SK287761B6 . 2011-09-05

机译：IL-18抑制剂的用途，含有编码IL-18抑制剂序列的表达载体的用途，该载体在细胞中诱导和/或扩增IL-18抑制剂的内源性生产的用途以及使用产生IL的细胞-18抑制剂

相关主题

U17/snR30 Is a Ubiquitous snoRNA with Two Conserved Sequence Motifs Essential for 18S rRNA Production

摘要

著录项

相似文献

相关主题

期刊订阅