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首页> 外文期刊>Molecular and Cellular Biology >Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media
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Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media

机译：酿酒酵母短期厌氧菌过程中转录组的动态重塑：半乳糖和葡萄糖培养基中Msn2和/或Msn4和其他因素的差异反应和作用。

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In contrast to previous steady-state analyses of the O2-responsive transcriptome, here we examined the dynamics of the response to short-term anaerobiosis (2 generations) in both catabolite-repressed (glucose) and derepressed (galactose) cells, assessed the specific role that Msn2 and Msn4 play in mediating the response, and identified gene networks using a novel clustering approach. Upon shifting cells to anaerobic conditions in galactose medium, there was an acute (～10 min) yet transient (<45 min) induction of Msn2- and/or Msn4-regulated genes associated with the remodeling of reserve energy and catabolic pathways during the switch from mixed respiro-fermentative to strictly fermentative growth. Concomitantly, MCB- and SCB-regulated networks associated with the G1/S transition of the cell cycle were transiently down-regulated along with rRNA processing genes containing PAC and RRPE motifs. Remarkably, none of these gene networks were differentially expressed when cells were shifted in glucose, suggesting that a metabolically derived signal arising from the abrupt cessation of respiration, rather than O2 deprivation per se, elicits this “stress response.” By ～0.2 generation of anaerobiosis in both media, more chronic, heme-dependent effects were observed, including the down-regulation of Hap1-regulated networks, derepression of Rox1-regulated networks, and activation of Upc2-regulated ones. Changes in these networks result in the functional remodeling of the cell wall, sterol and sphingolipid metabolism, and dissimilatory pathways required for long-term anaerobiosis. Overall, this study reveals that the acute withdrawal of oxygen can invoke a metabolic state-dependent “stress response” but that acclimatization to oxygen deprivation is a relatively slow process involving complex changes primarily in heme-regulated gene networks.
机译：与以前的O 2 反应性转录组的稳态分析相反，在这里我们研究了分解代谢物抑制（葡萄糖）和去抑制的对短期厌氧菌反应（2代）的动力学。 （半乳糖）细胞，评估Msn2和Msn4在介导反应中的特定作用，并使用新型聚类方法鉴定基因网络。在半乳糖培养基中将细胞转移至厌氧条件后，会在转换过程中对Msn2和/或Msn4调控基因进行急性（〜10分钟）但瞬时（<45分钟）诱导，与储备能量和分解代谢途径的重塑有关从混合呼吸发酵到严格发酵生长。同时，与细胞周期的G 1 / S转变相关的MCB和SCB调控网络与包含PAC和RRPE模体的rRNA加工基因一起被瞬时下调。值得注意的是，当细胞在葡萄糖中移动时，这些基因网络都没有差异表达，这表明由呼吸突然停止而不是由O 2 本身引起的代谢产生的信号引起了这种“压力”。响应。”通过在两种培养基中产生约0.2的厌氧菌，可以观察到更多的慢性血红素依赖性效应，包括Hap1调控网络的下调，Rox1调控网络的去抑制和Upc2调控网络的激活。这些网络的变化导致细胞壁的功能重塑，固醇和鞘脂代谢，以及长期厌氧菌所需的异化途径。总的来说，这项研究表明，氧气的突然撤除可以引起代谢状态依赖性的“应激反应”，但是适应缺氧是一个相对缓慢的过程，主要涉及血红素调节基因网络中的复杂变化。

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