• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex
【24h】

Replication Protein A-Directed Unloading of PCNA by the Ctf18 Cohesion Establishment Complex

机译：Ctf18内聚建立复合体复制蛋白A指导PCNA的卸载。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The replication clamp PCNA is loaded around DNA by replication factor C (RFC) and functions in DNA replication and repair. Regulated unloading of PCNA during the progression and termination of DNA replication may require additional factors. Here we show that a Saccharomyces cerevisiae complex required for the establishment of sister chromatid cohesion functions as an efficient unloader of PCNA. Unloading requires ATP hydrolysis. This seven-subunit Ctf18-RFC complex consists of the four small subunits of RFC, together with Ctf18, Dcc1, and Ctf8. Ctf18-RFC was also a weak loader of PCNA onto naked template-primer DNA. However, when the single-stranded DNA template was coated by the yeast single-stranded DNA binding protein replication protein A (RPA) but not by a mutant form of RPA or a heterologous single-stranded DNA binding protein, both binding of Ctf18-RFC to substrate DNA and loading of PCNA were strongly inhibited, and unloading predominated. Neither yeast RFC itself nor two other related clamp loaders, containing either Rad24 or Elg1, catalyzed significant unloading of PCNA. The Dcc1 and Ctf8 subunits of Ctf18-RFC, while required for establishing sister chromatid cohesion in vivo, did not function specifically in PCNA unloading in vitro, thereby separating the functionality of the Ctf18-RFC complex into two distinct paths.
机译：复制钳PCNA通过复制因子C（RFC）加载到DNA周围，并在DNA复制和修复中起作用。在DNA复制的进行和终止过程中PCNA的卸荷可能需要其他因素。在这里，我们表明建立姐妹染色单体内聚力所需的啤酒酵母复合物可作为PCNA的有效卸荷物。卸货需要ATP水解。这个由七个子单元构成的Ctf18-RFC复合体由RFC的四个小子单元以及Ctf18，Dcc1和Ctf8组成。 Ctf18-RFC还是PCNA裸模板引物DNA的弱加载者。但是，当单链DNA模板被酵母单链DNA结合蛋白复制蛋白A（RPA）覆盖，而不被RPA的突变形式或异源单链DNA结合蛋白覆盖时，Ctf18-RFC的结合底物DNA的PCNA和PCNA的负载被强烈抑制，卸载占主导。酵母RFC本身或其他两个包含Rad24或Elg1的相关钳式装载器都没有催化PCNA的显着卸载。 Ctf18-RFC的Dcc1和Ctf8亚基虽然在体内建立姐妹染色单体的内聚力所必需，但在体外PCNA卸载中没有特定功能，因此将Ctf18-RFC复合物的功能分为两个不同的途径。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号