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首页> 外文期刊>Molecular and Cellular Biology >Evolutionarily Conserved Function of RRP36 in Early Cleavages of the Pre-rRNA and Production of the 40S Ribosomal Subunit
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Evolutionarily Conserved Function of RRP36 in Early Cleavages of the Pre-rRNA and Production of the 40S Ribosomal Subunit

机译：RRP36在pre-rRNA的早期切割和40S核糖体亚基生产中的进化保守功能。

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Ribosome biogenesis in eukaryotes is a major cellular activity mobilizing the products of over 200 transcriptionally coregulated genes referred to as the rRNA and ribosome biosynthesis regulon. We investigated the function of an essential, uncharacterized gene of this regulon, renamed RRP36. We show that the Rrp36p protein is nucleolar and interacts with 90S and pre-40S preribosomal particles. Its depletion affects early cleavages of the 35S pre-rRNA and results in a rapid decrease in mature 18S rRNA levels. Rrp36p is a novel component of the 90S preribosome, the assembly of which has been suggested to result from the stepwise incorporation of several modules, including the tUTP/UTP-A, PWP2/UTP-B, and UTP-C subcomplexes. We show that Rrp36p depletion does not impair the incorporation of these subcomplexes and the U3 small nucleolar RNP into preribosomes. In contrast, depletion of components of the UTP-A or UTP-B modules, but not Rrp5p, prevents Rrp36p recruitment and reduces its accumulation levels. In parallel, we studied the human orthologue of Rrp36p in HeLa cells, and we show that the function of this protein in early cleavages of the pre-rRNA has been conserved through evolution in eukaryotes.
机译：真核生物中的核糖体生物发生是一种主要的细胞活性，动员了200多种转录共调控基因的产物，这些基因被称为rRNA和核糖体生物合成调节子。我们研究了该调节子的重要，未表征的基因的功能，改名为 RRP36 。我们表明，Rrp36p蛋白是核仁，并与90S和40S前核糖体颗粒相互作用。它的耗竭会影响35S pre-rRNA的早期切割，并导致成熟的18S rRNA水平迅速降低。 Rrp36p是90S前核糖体的新型成分，已提出其组装是由于逐步整合了多个模块（包括tUTP / UTP-A，PWP2 / UTP-B和UTP-C亚复合物）而导致的。我们表明，Rrp36p耗竭不会损害这些亚复合体和U3小核仁RNP纳入原核糖体。相反，消耗UTP-A或UTP-B模块的组件而不消耗Rrp5p可以防止Rrp36p募集并降低其积累水平。平行地，我们研究了人在HeLa细胞中的Rrp36p直向同源物，并且我们证明了该蛋白在pre-rRNA的早期切割中的功能已通过真核生物的进化得以保存。

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