The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.

H R Waterham; Y de Vries; K A Russel; W Xie; M Veenhuis; J M Cregg

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The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.

机译：巴斯德毕赤酵母PER6基因产物是过氧化物酶体生物发生必不可少的过氧化物酶体整合膜蛋白，与Zellweger综合征蛋白PAF-1具有序列相似性。

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We report the cloning of PER6, a gene essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. The PER6 sequence predicts that its product Per6p is a 52-kDa polypeptide with the cysteine-rich C3HC4 motif. Per6p has significant overall sequence similarity with the human peroxisome assembly factor PAF-1, a protein that is defective in certain patients suffering from the peroxisomal disorder Zellweger syndrome, and with car1, a protein required for peroxisome biogenesis and caryogamy in the filamentous fungus Podospora anserina. In addition, the C3HC4 motif and two of the three membrane-spanning segments predicted for Per6p align with the C3HC4 motifs and the two membrane-spanning segments predicted for PAF-1 and car1. Like PAF-1, Per6p is a peroxisomal integral membrane protein. In methanol- or oleic acid-induced cells of per6 mutants, morphologically recognizable peroxisomes are absent. Instead, peroxisomal remnants are observed. In addition, peroxisomal matrix proteins are synthesized but located in the cytosol. The similarities between Per6p and PAF-1 in amino acid sequence and biochemical properties, and between mutants defective in their respective genes, suggest that Per6p is the putative yeast homolog of PAF-1.

机译：我们报道了PER6的克隆，PER6是甲基营养型酵母巴斯德毕赤酵母中过氧化物酶体生物发生必不可少的基因。 PER6序列预测其产物Per6p是具有富含半胱氨酸的C3HC4基序的52 kDa多肽。 Per6p与人类过氧化物酶体装配因子PAF-1（一种在某些患有过氧化物酶体疾病Zellweger综合征的患者中存在缺陷的蛋白质）以及与car1（丝状真菌Podospora anserina中的过氧化物酶体生物发生和同卵生殖所必需的蛋白质）具有显着的总体序列相似性。。此外，C3HC4主题和为Per6p预测的三个跨膜片段中的两个与C3HC4主题和为PAF-1和car1预测的两个跨膜片段对齐。与PAF-1一样，Per6p是过氧化物酶体整合膜蛋白。在per6突变体的甲醇或油酸诱导的细胞中，缺乏形态学上可识别的过氧化物酶体。相反，观察到过氧化物酶体残留。另外，过氧化物酶体基质蛋白被合成但位于胞质溶胶中。 Per6p和PAF-1在氨基酸序列和生化特性上以及在各自基因中有缺陷的突变体之间的相似性表明，Per6p是PAF-1的推定酵母同源物。

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《Molecular and Cellular Biology》 |1996年第5期|共10页
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H R Waterham; Y de Vries; K A Russel; W Xie; M Veenhuis; J M Cregg;
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中图分类细胞生物学;
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1. The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family. [J] . D McCollum, E Monosov, S Subramani Journal of cell biology . 1993,第4期

机译：巴斯德毕赤酵母的pas8突变体表现出Zellweger综合征细胞的过氧化物酶体蛋白输入缺陷-PAS8蛋白与COOH末端三肽过氧化物酶体靶向信号结合，并且是TPR蛋白家族的成员。
2. PAS3, a Saccharomyces cerevisiae gene encoding a peroxisomal integral membrane protein essential for peroxisome biogenesis. [J] . J H?hfeld, M Veenhuis, W H Kunau Journal of cell biology . 1991,第6期

机译：PAS3，一种酿酒酵母基因，编码过氧化物酶体生物发生所必需的过氧化物酶体整合膜蛋白。
3. The 22-kD Peroxisomal Integral Membrane Protein in Zellweger Syndrome|[mdash]|Presence, Abundance, and Association with a Peroxisomal Thiolase Precursor Protein [J] . Jutta G?rtner, Winston W Chen, Richard I Kelley, Pediatric Research . 1991,第2期

机译：Zellweger综合征中的22 kD过氧化物酶体整体膜蛋白| [mdash] |存在，丰度以及与过氧化物酶体硫羟酶前体蛋白的关联
4. The use of insertion sequences to analyse gene function in thiobacilus ferrooxidans: a case study involving cytochrome c-type biogenesis proteins in iron oxidation [C] . D.S.Holmes, E.Jedlicki, M.E.Cabrejos, International biohydrometallurgy symposium;IBS'99 . 1999

机译：利用插入序列分析氧化硫硫杆菌的基因功能：一个涉及铁氧化中细胞色素c型生物合成蛋白的案例研究
5. Novel genetic selections for peroxisome biogenesis mutants (pex) and the isolation and characterization of PEX14 and Pex14p in Pichia pastoris [D] . Johnson, Monique Ami 2000

机译：过氧化物酶体生物发生突变体（pex）的新型遗传选择以及巴斯德毕赤酵母中PEX14和Pex14p的分离和表征
6. The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1. [O] . H R Waterham, Y de Vries, K A Russel, 1996

机译：巴斯德毕赤酵母PER6基因产物是过氧化物酶体生物合成必不可少的过氧化物酶体整合膜蛋白与Zellweger综合征蛋白PAF-1具有序列相似性。
7. The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1. [O] . Waterham, H R, de Vries, Y, Russel, K A, 1996

机译：巴斯德毕赤酵母PER6基因产物是过氧化物酶体生物合成必不可少的过氧化物酶体整合膜蛋白，与Zellweger综合征蛋白PAF-1具有序列相似性。
8. Transcriptional Regulation of Genes Encoding Proteins Involved in Peroxisome Biogenesis in Yeast [R] . Einerhand, A. W. C. 1992

机译：酵母中过氧化物酶体生物合成中蛋白质编码基因的转录调控

The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.

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