...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)
【24h】

Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4)

机译：酿酒酵母Upf2蛋白和Upf3蛋白（秀丽隐杆线虫SMG-4）的人类直系同源物的鉴定和表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nonsense-mediated mRNA decay (NMD), also called mRNA surveillance, is an important pathway used by all organisms that have been tested to degrade mRNAs that prematurely terminate translation and, as a consequence, eliminate the production of aberrant proteins that could be potentially harmful. In mammalian cells, NMD appears to involve splicing-dependent alterations to mRNA as well as ribosome-associated components of the translational apparatus. To date, human (h) Upf1 protein (p) (hUpf1p), a group 1 RNA helicase named after itsSaccharomyces cerevisiae orthologue that functions in both translation termination and NMD, has been the only factor shown to be required for NMD in mammalian cells. Here, we describe human orthologues to S. cerevisiae Upf2p and S. cerevisiae Upf3p (Caenorhabditis elegans SMG-4) based on limited amino acid similarities. The existence of these orthologues provides evidence for a higher degree of evolutionary conservation of NMD than previously appreciated. Interestingly, human orthologues toS. cerevisiae Upf3p (C. elegans SMG-4) derive from two genes, one of which is X-linked and both of which generate multiple isoforms due to alternative pre-mRNA splicing. We demonstrate using immunoprecipitations of epitope-tagged proteins transiently produced in HeLa cells that hUpf2p interacts with hUpf1p, hUpf3p-X, and hUpf3p, and we define the domains required for the interactions. Furthermore, we find by using indirect immunofluorescence that hUpf1p is detected only in the cytoplasm, hUpf2p is detected primarily in the cytoplasm, and hUpf3p-X localizes primarily to nuclei. The finding that hUpf3p-X is a shuttling protein provides additional indication that NMD has both nuclear and cytoplasmic components.
机译：无义介导的mRNA衰减（NMD），也称为mRNA监测，是所有经过测试可降解过早终止翻译的mRNA的有机体使用的重要途径，因此消除了可能有害的异常蛋白质的产生。 。在哺乳动物细胞中，NMD似乎涉及翻译装置的mRNA以及与核糖体相关的成分的剪接依赖性改变。迄今为止，人类（h）Upf1蛋白（p）（hUpf1p）是一种第1组RNA解旋酶，以其在酿酒酵母中的直向同源物命名，在翻译终止和NMD中均起着作用，是唯一显示出以下因素的因子是哺乳动物细胞中NMD所必需的。在这里，我们将人类直向同源物描述为 S。啤酒 Upf2p和 S。基于有限的氨基酸相似性的啤酒酵母Upf3p（秀丽隐杆线虫 SMG-4）。这些直向同源物的存在为NMD的进化保守性提供了比以前认识到更高的证据。有趣的是，人类与 S的直系同源物。酿酒酵母Upf3p（ C。elegans SMG-4）来源于两个基因，其中一个是X连锁的，并且由于mRNA的前期剪接，两个都产生多种同工型。我们证明了使用免疫沉淀作用在HeLa细胞中瞬时产生的抗原决定簇标记的蛋白上与hUpf1p，hUpf3p-X和hUpf3p相互作用的HeLa细胞，并定义了相互作用所需的结构域。此外，我们发现通过使用间接免疫荧光只能在细胞质中检测到hUpf1p，主要在细胞质中检测到hUpf2p，而hUpf3p-X主要定位于细胞核。 hUpf3p-X是一种穿梭蛋白的发现进一步表明NMD同时具有核和细胞质成分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号