Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes

Chunqi Ma; Cathy Cummings; X. Johné Liu

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Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes

机译：爪蟾卵母细胞减数分裂I-减数分裂II过渡期间Aurora-A激酶的双相激活

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Xenopus Aurora-A (also known as Eg2) is a member of the Aurora family of mitotic serine/threonine kinases. In Xenopus oocytes, Aurora-A phosphorylates and activates a cytoplasmic mRNA polyadenylation factor (CPEB) and therefore plays a pivotal role in MOS translation. However, hyperphosphorylation and activation of Aurora-A appear to be dependent on maturation-promoting factor (MPF) activation. To resolve this apparent paradox, we generated a constitutively activated Aurora-A by engineering a myristylation signal at its N terminus. Injection of Myr-Aurora-A mRNA induced germinal vesicle breakdown (GVBD) with the concomitant activation of MOS, mitogen-activated protein kinase, and MPF. Myr-Aurora-A-injected oocytes, however, appeared to arrest in meiosis I with high MPF activity and highly condensed, metaphase-like chromosomes but no organized microtubule spindles. No degradation of CPEB or cyclin B2 was observed following GVBD in Myr-Aurora-A-injected oocytes. In the presence of progesterone, the endogenous Aurora-A became hyperphosphorylated and activated at the time of MPF activation. Following GVBD, Aurora-A was gradually dephosphorylated and inactivated before it was hyperphosphorylated and activated again. This biphasic pattern of Aurora-A activation mirrored that of MPF activation and hence may explain meiosis I arrest by the constitutively activated Myr-Aurora-A.

机译：非洲爪蟾 Aurora-A（也称为Eg2）是Aurora有丝分裂丝氨酸/苏氨酸激酶家族的成员。在非洲爪蟾卵母细胞中，Aurora-A磷酸化并激活细胞质mRNA聚腺苷酸化因子（CPEB），因此在 MOS 翻译中起关键作用。但是，Aurora-A的过度磷酸化和激活似乎取决于成熟促进因子（MPF）的激活。为了解决这一明显的矛盾，我们通过在其N端工程化肉豆蔻化信号来生成组成型激活的Aurora-A。注入Myr-Aurora-A mRNA会导致生小泡破裂（GVBD），同时伴随MOS激活，丝裂原激活的蛋白激酶和MPF。然而，注入Myr-Aurora-A的卵母细胞似乎停滞在具有高MPF活性和高度浓缩的中期样染色体的减数分裂I中，但没有组织的微管纺锤体。在注入Myr-Aurora-A的卵母细胞中进行GVBD后，未观察到CPEB或细胞周期蛋白B2的降解。在孕酮存在下，内源性Aurora-A在MPF激活时被过度磷酸化并被激活。在GVBD之后，Aurora-A逐渐被去磷酸化和失活，然后被超磷酸化并再次被激活。 Aurora-A激活的这种双相模式与MPF激活相类似，因此可以解释减数分裂I被组成型激活的Myr-Aurora-A阻滞。 展开▼

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《Molecular and Cellular Biology》 |2003年第5期|共14页

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Chunqi Ma; Cathy Cummings; X. Johné Liu;
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中图分类细胞生物学;

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1. Regulation of Separase in meiosis: Separase is activated at the metaphase I-II transition in Xenopus oocytes during meiosis. [J] . Fan HY, Sun QY, Zou H Cell cycle . 2006,第2期

机译：减数分裂中Separase的调节：在减数分裂过程中，爪蟾卵母细胞的中期I-II过渡激活Separase。

2. Emi1 class of proteins regulate entry into meiosis and the meiosis I to meiosis II transition in Xenopus oocytes. [J] . Tung JJ, Jackson PK Cell cycle . 2005,第3期

机译：Emi1类蛋白质可调节非洲爪蟾卵母细胞进入减数分裂的过程以及减数分裂I向减数分裂II的转变。

3. Erp1/Emi2 is essential for the meiosis I to meiosis II transition in Xenopus oocytes. [J] . Ohe M, Inoue D, Kanemori Y, Developmental biology . 2007,第1期

机译：Erp1 / Emi2对于非洲爪蟾卵母细胞从减数分裂I到减数分裂II的转变至关重要。

4. IMMUNOGOLD ELECTRON MICROSCOPIC STUDY ON ENZYMES INVOLVED IN THE PRODUCTION OF MEIOSIS ACTIVATING STEROLS WITHIN THE TESTIS [C] . D. Jezek, M. Cotman, Lj. Banek, 5th Multinational Congress on Electron Microscopy Sep 20-25, 2001 Lecce, ITALY . 2001

机译：睾丸中减数分裂活性甾醇生产中涉及的酶的免疫金电子显微镜研究

5. The role of protein kinase B/Akt signaling in the resumption of meiosis in oocytes from Xenopus laevis. [D] . Andersen, Carsten Bo. 2002

机译：蛋白激酶B / Akt信号传导在非洲爪蟾卵母细胞恢复减数分裂中的作用。

6. Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes [O] . Chunqi Ma, Cathy Cummings, X. Johné Liu 2003

机译：爪蟾卵母细胞减数分裂I-减数分裂II过渡期间Aurora-A激酶的双相激活

7. Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes [O] . Ma, Chunqi, Cummings, Cathy, Liu, X. Johné 2003

机译：爪蟾卵母细胞减数分裂I-减数分裂II过渡期间Aurora-A激酶的双相激活

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2. 正常培养条件下绵羊卵母细胞减数分裂从中期I至中期III的动态变化过程 [J] . 李欣欣 ,白春玲 ,魏著英 . 生物技术通报 . 2016,第005期

3. 小鼠卵母细胞减数分裂期间组蛋白乙酰化及其对卵母细胞成熟质量的影响 [J] . 王婧 ,李静心 ,孟春花 . 江苏农业学报 . 2014,第002期

4. 用Polscope观察人类卵母细胞中MII期减数分裂的纺锤体能预测ICSI后胚胎发育能力 [J] . 金海霞 ,孙莹璞 . 国际生殖健康/计划生育杂志 . 2003,第004期

5. 钙调蛋白依赖性蛋白激酶Ⅱ在小鼠卵母细胞减数分裂恢复中的作用 [J] . 郝肖琼 ,付旭阳 ,崔艳颖 . 包头医学院学报 . 2019,第011期

6. 牛卵母细胞第一次减数分裂比第二次减数分裂对Cyclin B1表达量上升更敏感 [C] . 刘文强 ,雷安民 . 第五次全国动物生物技术学术研讨会 . 2010

7. Aurora-A在猪卵母细胞减数分裂中的作用与机制研究 [A] . 杨笑柳 . 2016

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2. 一种体外保存/培养卵母细胞以维持卵母细胞减数分裂停滞的方法 [P] . 中国专利： CN102258005B . 2013.07.17

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机译：刺激发芽细胞减数分裂的方法以及使用引起减数分裂活化的内源性物质积聚的化合物的用途

4. Meiosis arrest in oocytes in vitro [P] . 外国专利： US2008311656A1 . 2008-12-18

机译：卵母细胞体外减数分裂阻滞

5. Meiosis arrest in human oocytes in vitro [P] . 外国专利： US2006051863A1 . 2006-03-09

机译：人卵母细胞体外减数分裂阻滞

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Biphasic Activation of Aurora-A Kinase during the Meiosis I- Meiosis II Transition in Xenopus Oocytes

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