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首页> 外文期刊>Molecular and Cellular Biology >Yeast Shuttling SR Proteins Npl3p, Gbp2p, and Hrb1p Are Part of the Translating mRNPs, and Npl3p Can Function as a Translational Repressor
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Yeast Shuttling SR Proteins Npl3p, Gbp2p, and Hrb1p Are Part of the Translating mRNPs, and Npl3p Can Function as a Translational Repressor

机译：酵母穿梭SR蛋白Npl3p，Gbp2p和Hrb1p是翻译mRNP的一部分，并且Npl3p可以充当翻译阻遏物

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摘要

A major challenge in current molecular biology is to understand how sequential steps in gene expression are coupled. Recently, much attention has been focused on the linkage of transcription, processing, and mRNA export. Here we describe the cytoplasmic rearrangement for shuttling mRNA binding proteins in Saccharomyces cerevisiae during translation. While the bulk of Hrp1p, Nab2p, or Mex67p is not associated with polysome containing mRNAs, significant amounts of the serine/arginine (SR)-type shuttling mRNA binding proteins Npl3p, Gbp2p, and Hrb1p remain associated with the mRNA-protein complex during translation. Interestingly, a prolonged association of Npl3p with polysome containing mRNAs results in translational defects, indicating that Npl3p can function as a negative translational regulator. Consistent with this idea, a mutation in NPL3 that slows down translation suppresses growth defects caused by the presence of translation inhibitors or a mutation in eIF5A. Moreover, using sucrose density gradient analysis, we provide evidence that the import receptor Mtr10p, but not the SR protein kinase Sky1p, is involved in the timely regulated release of Npl3p from polysome-associated mRNAs. Together, these data shed light onto the transformation of an exporting to a translating mRNP.
机译：当前分子生物学的主要挑战是了解基因表达的顺序步骤是如何耦合的。最近，许多注意力集中在转录，加工和mRNA出口的联系上。在这里，我们描述了在翻译过程中穿梭于酿酒酵母中的mRNA结合蛋白的胞质重排。虽然大部分Hrp1p，Nab2p或Mex67p与含有多核糖体的mRNA不相关，但是在翻译过程中，大量的丝氨酸/精氨酸（SR）型穿梭mRNA结合蛋白Npl3p，Gbp2p和Hrb1p仍与mRNA-蛋白质复合体相关。有趣的是，Npl3p与含有mRNA的多核糖体的长时间结合会导致翻译缺陷，表明Npl3p可以充当负翻译调节因子。与这个想法一致的是， NPL3 中的一个减慢翻译的突变抑制了翻译抑制剂的存在或eIF5A突变引起的生长缺陷。此外，使用蔗糖密度梯度分析，我们提供了证据，即进口受体Mtr10p而不是SR蛋白激酶Sky1p参与了Npl3p从多核糖体相关mRNA的及时调节释放。这些数据加在一起，阐明了从出口到翻译mRNP的转化。

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