Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages

Philipp Oberdoerffer; Chryssa Kanellopoulou; Vigo Heissmeyer; Corinna Paeper; Christine Borowski; Iannis Aifantis; Anjana Rao; Klaus Rajewsky

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Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages

机译：小鼠造血系统中RNA干扰的效率在细胞类型和发育阶段之间变化

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RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is more widely applicable than classical gene targeting and allows for the simultaneous inactivation of several homologous genes with a single transgene. Recently, RNAi has been used for conditional and conventional gene inactivation in mice. Unlike gene targeting, RNAi is a dynamic process, and its efficiency may vary both between cell types and throughout development. Here we demonstrate that RNAi can be used to target three separately encoded isoforms of the bcl-2 family gene bfl-1/A1 in a conditional manner in mice. The extent of gene inactivation varies between different cell types and is least efficient in mature lymphocytes. Our data suggest that RNAi is affected by factors beyond small interfering RNA-mRNA stoichiometry.

机译：RNA干扰（RNAi）是一种天然存在的转录后基因沉默机制，已被用作遗传工具，用于各种生物的功能丧失研究。它比传统的基因靶向方法更广泛地应用，并允许通过单个转基因同时灭活几个同源基因。最近，RNAi已用于小鼠的条件性和常规基因失活。与基因靶向不同，RNAi是一个动态过程，其效率可能在细胞类型之间以及整个发育过程中都不同。在这里，我们证明RNAi可以有条件地在小鼠中用于靶向 bcl-2 家族基因 bfl-1 / A1 的三个单独编码的同工型。基因失活的程度在不同细胞类型之间变化，并且在成熟淋巴细胞中效率最低。我们的数据表明，RNAi受超出小干扰RNA-mRNA化学计量的因素影响。 展开▼

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《Molecular and Cellular Biology》 |2005年第10期|共10页

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Philipp Oberdoerffer; Chryssa Kanellopoulou; Vigo Heissmeyer; Corinna Paeper; Christine Borowski; Iannis Aifantis; Anjana Rao; Klaus Rajewsky;
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中图分类细胞生物学;

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Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages

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