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NuA4-Directed Chromatin Transactions throughout the Saccharomyces cerevisiae Genome

机译：整个酿酒酵母基因组中由NuA4指导的染色质交易

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Two of the major histone acetyltransferases in Saccharomyces cerevisiae are NuA4 and SAGA, which acetylate histones H4 and H3, respectively. Acetylated H3 and H4 tails have been implicated in binding bromodomain proteins, including Bdf1. Bdf1 interacts with the general transcription factor TFIID, which might promote preinitiation complex (PIC) assembly. Bdf1 also interacts with the SWR complex (SWR-C). SWR-C is responsible for the deposition of the histone H2A variant H2A.Z. The placement of these interactions into a connected pathway of PIC assembly has not been fully established. Moreover, it is not known how widespread and how variable such a pathway might be on a genomic scale. Here we provide genomic evidence for S. cerevisiae that PIC assembly (TFIID occupancy) and chromatin remodeling (SWR-C and H2A.Z occupancy) are linked in large part to NuA4-directed H4 acetylation and subsequent Bdf1 binding, rather than through SAGA-directed H3 acetylation. Bdf1 and its homolog Bdf2 tend to have distinct locations in the genome. However, the deletion of BDF1 leads to the accumulation of Bdf2 at Bdf1-vacated sites. Thus, while Bdf1 and Bdf2 are at least partially redundant in function, their functions in the genome are geographically distinct.
机译：酿酒酵母中的两个主要组蛋白乙酰转移酶是NuA4和SAGA，它们分别乙酰化组蛋白H4和H3。乙酰化的H3和H4尾巴已经牵涉到结合溴结构域蛋白，包括Bdf1。 Bdf1与一般转录因子TFIID相互作用，这可能会促进预启动复合物（PIC）组装。 Bdf1还与SWR复合体（SWR-C）交互。 SWR-C负责组蛋白H2A变体H2A.Z的沉积。这些交互作用到PIC组件的连接路径中的位置尚未完全确定。而且，还不知道这种途径在基因组规模上有多广泛和如何可变。在这里，我们提供了 S的基因组证据。酿酒酵母中，PIC组装（TFIID占用）和染色质重塑（SWR-C和H2A.Z占用）在很大程度上与NuA4指导的H4乙酰化和随后的Bdf1结合有关，而不是通过SAGA指导的H3乙酰化。 Bdf1及其同源物Bdf2在基因组中倾向于具有不同的位置。但是， BDF1 的缺失会导致Bdf2在Bdf1缺失的位点积累。因此，尽管Bdf1和Bdf2在功能上至少部分是多余的，但它们在基因组中的功能在地理上是不同的。

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