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A+U-Rich Instability Elements Differentially Activate 5′-3′ and 3′-5′ mRNA Decay

机译：富含A + U的不稳定性元件差异激活5'-3'和3'-5'mRNA衰变

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The A+U-rich elements (or AREs) are cis-acting sequences that activate rapid mRNA decay, yet the overall polarity of this process is unknown. The current study describes an unbiased approach to this using the Invader RNA assay (Third Wave Technologies, Inc.) to quantify the decay of each of the three exons of human β-globin mRNA without added instability elements or with the AREs from c-fos or granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in the 3′ untranslated region. Each of these genes under tetracycline operator control was stably transfected into cells, and β-globin mRNA was quantified with exon-specific probes following transcription termination. There was little overall evidence for polarity in stable mRNA decay. Adding the c-fos ARE activated rapid and simultaneous decay from both ends of the mRNA. In contrast, the GM-CSF ARE activated decay primarily from the mRNA 5′ end. These data were supported by reciprocal RNA interference knockdowns, and we present evidence that the 5′-3′ and 3′-5′ decay pathways are functionally linked.

机译：富含A + U的元件（或ARE）是顺式作用序列，可激活mRNA的快速衰减，但该过程的整体极性尚不清楚。当前的研究描述了一种使用Invader RNA分析（Third Wave Technologies，Inc.）的无偏方法，以量化人β-珠蛋白mRNA的三个外显子中每个外显子的衰变，而无需添加不稳定性元素或使用c- < 3'非翻译区中的em> fos 或粒细胞巨噬细胞集落刺激因子（GM-CSF）mRNA。将这些基因在四环素操作员控制下稳定地转染到细胞中，并在转录终止后用外显子特异性探针对β-珠蛋白mRNA进行定量。稳定的mRNA衰减中极性的总体证据很少。加入c- fos ARE可以从mRNA的两端快速而同时地衰减。相反，GM-CSF ARE激活的衰变主要从mRNA 5'端开始。这些数据由相互的RNA干扰敲低支持，我们提供了5'-3'和3'-5'衰变途径在功能上相关的证据。 展开▼

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《Molecular and Cellular Biology》 |2007年第8期|共9页

作者
Elizabeth L. Murray; Daniel R. Schoenberg;
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中图分类细胞生物学;

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专利

1. Upf1/Upf2 Regulation of 3′ Untranslated Region Splice Variants of AUF1 Links Nonsense-Mediated and A+U-Rich Element-Mediated mRNA Decay [J] . Lili Banihashemi, Gerald M. Wilson, Neha Das, Molecular and Cellular Biology . 2006,第23期

机译：AUF1的3'非翻译区剪接变体的Upf1 / Upf2调节链接无义介导和A + U富元素介导的mRNA衰变

2. The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae The decapping activator Edc3 and the Q/N-rich domain of Lsm4 function together to enhance mRNA stability and alter mRNA decay pathway dependence in Saccharomyces cerevisiae [J] . Vidya Balagopal, Mridula Muppavarapu, Tracy Nissan, Biology Open . 2016,第10期

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3. A cytokine mRNA-destabilizing element that is structurally and functionally distinct from A+U-rich elements [J] . Cheryl Y. Brown, Cathy A. Lagnado, Gregory J. Goodall Proceedings of the National Academy of Sciences of the United States of America . 1996,第24期

机译：细胞因子mRNA不稳定元件，在结构和功能上与富含A + U的元件不同

4. Boundary element slope instability modeling of Corinth Canal, Greece due to nearby fault activation [C] . G.-A. Tselentis, F. Gkika International Conference on Computer Modelling and Experimental Measurements, Engineering and Management of Seas and Coastal Regions(Coastal Engineering VII); ; Algarve(PT) . -1

机译：希腊科林斯运河因附近断层活化而引起的边界元边坡失稳模型

5. Selective translation and regulated decay of yeast mRNAs in response to carbon source: cis elements and trans factors [D] . de la Cruz, Bernard Jorque 2000

机译：响应碳源的酵母mRNA的选择性翻译和调控的衰变：顺式元素和反式因子

6. A+U-Rich Instability Elements Differentially Activate 5′-3′ and 3′-5′ mRNA Decay [O] . Elizabeth L. Murray, Daniel R. Schoenberg 2007

机译：富含A + U的不稳定性元件差异激活5-3和3-5mRNA衰变

7. A+U-Rich Instability Elements Differentially Activate 5′-3′ and 3′-5′ mRNA Decay▿ [O] . Murray, Elizabeth L., Schoenberg, Daniel R. 2007

机译：富含A + U的不稳定性元件差异激活5'-3'和3'-5'mRNA衰变▿

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7. 粘细菌mRNA差异表达法研究及耐盐粘球菌HW-1的mRNA差异表达分析 [A] . 龚勋 . 2005

1. DRD1 mRNA 3端非编码区ARE元件通过稳定DRD1 mRNA上调其表达 [P] . 中国专利： CN101928706A . 2010-12-29

2. 一种差异核酸酶切方法及其在LC-MS法检测mRNA内部修饰中的应用 [P] . 中国专利： CN107460219B . 2020.04.10

3. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . 外国专利： BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法

4. Control primer annealing to improve the specificity of annealing in nucleic acid amplification, kit, methods for amplifying nucleic acid sequences of DNA and sequence of target nucleic acid or a mixture of nucleic acids, to detect the complementari Dade of DNA to mRNA differentially expressed in two or more samples of nucleic acid.To amplify a fragment of the cDNA target quickly, to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs to amplify rather than a sequence of nucleotide al VO at the same time, to produce a digital printing gDNA DNA, and RNA in a sample of mRNA.To identify segments of homology is conserved in a multigene family from a sample of mRNA, to identify a variation of nucleotide in a target nucleic acid, for mutagenesis in a target nucleic acid, and use of the initiator [P] . 外国专利： BRPI0214741B1 . 2015-05-12

机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途

5. Gloucose-regulated mRNA instability element [P] . 外国专利： US6852529B1 . 2005-02-08

机译：葡萄糖调节的mRNA稳定元件

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