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首页> 外文期刊>Molecular and Cellular Biology >Rtp1p Is a Karyopherin-Like Protein Required for RNA Polymerase II Biogenesis
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Rtp1p Is a Karyopherin-Like Protein Required for RNA Polymerase II Biogenesis

机译：Rtp1p是RNA聚合酶II生物发生所需的核转运蛋白样蛋白。

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The assembly and nuclear transport of RNA polymerase II (RNA pol II) are processes that require the participation of many auxiliary factors. In a yeast genetic screen, we identified a previously uncharacterized gene, YMR185w (renamed RTP1), which encodes a protein required for the nuclear import of RNA pol II. Using protein affinity purification coupled to mass spectrometry, we identified interactions between Rtp1p and members of the R2TP complex. Rtp1p also interacts, to a different extent, with several RNA pol II subunits. The pattern of interactions is compatible with a role for Rtp1p as an assembly factor that participates in the formation of the Rpb2/Rpb3 subassembly complex and its binding to the Rpb1p-containing subcomplex. Besides, Rtp1p has a molecular architecture characteristic of karyopherins, composed of HEAT repeats, and is able to interact with phenylalanine-glycine-containing nucleoporins. Our results define Rtp1p as a new component of the RNA pol II biogenesis machinery that plays roles in subunit assembly and likely in transport through the nuclear pore complex.
机译：RNA聚合酶II（RNA pol II）的组装和核转运是需要许多辅助因子参与的过程。在酵母遗传筛选中，我们鉴定了一个以前未鉴定的基因 YMR185w （重命名为 RTP1 ），该基因编码RNA pol II核导入所需的蛋白质。使用蛋白质亲和纯化与质谱联用，我们确定了Rtp1p与R2TP复合物成员之间的相互作用。 Rtp1p还与几种RNA pol II亚基相互作用，程度不同。相互作用的模式与Rtp1p作为装配因子的角色兼容，该装配因子参与Rpb2 / Rpb3子装配复合体的形成及其与包含Rpb1p的子复合体的结合。此外，Rtp1p具有由HEAT重复组成的核球蛋白的分子结构特征，并且能够与含苯丙氨酸-甘氨酸的核孔蛋白相互作用。我们的结果将Rtp1p定义为RNA pol II生物发生机制的新组成部分，该机制在亚基组装中可能发挥作用，并可能在通过核孔复合体的运输中发挥作用。

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