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首页> 外文期刊>Molecular and Cellular Biology >In Vivo Analysis of Developmentally and Evolutionarily Dynamic Protein-DNA Interactions Regulating Transcription of the Pgk2 Gene during Mammalian Spermatogenesis
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In Vivo Analysis of Developmentally and Evolutionarily Dynamic Protein-DNA Interactions Regulating Transcription of the Pgk2 Gene during Mammalian Spermatogenesis

机译：在哺乳动物精子发生过程中调节Pgk2基因转录的发育和进化动态蛋白质-DNA相互作用的体内分析。

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Transcription of the testis-specific Pgk2 gene is selectively activated in primary spermatocytes to provide a source of phosphoglycerate kinase that is critical to normal motility and fertility of mammalian spermatozoa. We examined dynamic changes in protein-DNA interactions at the Pgk2 gene promoter during murine spermatogenesis in vivo by performing genomic footprinting and chromatin immunoprecipitation assays with enriched populations of murine spermatogenic cells at stages prior to, during, and following transcription of this gene. We found that genes encoding the testis-specific homeodomain factor PBX4 and its coactivator, PREP1, are expressed in patterns that mirror expression of the Pgk2 gene and that these factors become bound to the Pgk2 enhancer in cells in which this gene is actively expressed. We therefore suggest that these factors, along with CREM and SP3, direct stage- and cell type-specific transcription of the Pgk2 gene during spermatogenesis. We propose that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene. This study provides insight into the developmentally dynamic establishment of tissue-specific protein-DNA interactions in vivo. It also allows us to speculate about the events that led to tissue-specific regulation of the Pgk2 gene during mammalian evolution.
机译：睾丸特异的 Pgk2 基因的转录在原代精子细胞中被选择性激活，以提供磷酸甘油酸激酶的来源，该磷酸甘油酸激酶对于哺乳动物精子的正常运动和受精至关重要。我们通过在小鼠精子发生之前，之中和之后的阶段进行基因组足迹和染色质免疫沉淀测定，通过研究小鼠精子发生的过程，研究了 Pgk2 基因启动子上蛋白质-DNA相互作用的动态变化。该基因的转录。我们发现，编码睾丸特异性同源结构域因子PBX4及其共激活因子PREP1的基因以镜像 Pgk2 基因表达的模式表达，并且这些因子与 Pgk2 结合。 em>该基因在其中活跃表达的细胞中的增强子。因此，我们建议这些因素以及CREM和SP3指导精子发生过程中 Pgk2 基因的阶段和细胞类型特异性转录。我们提出，PBX4及其辅激活物PREP1的结合是导致 Pgk2 基因组织特异性转录起始的限速步骤。这项研究提供了对体内组织特异性蛋白质-DNA相互作用发展动态建立的见解。它还使我们能够推测导致哺乳动物进化过程中 Pgk2 基因的组织特异性调控的事件。

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