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首页> 外文期刊>Molecular and Cellular Biology >Role of Nucleotide Binding in Septin-Septin Interactions and Septin Localization in Saccharomyces cerevisiae
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Role of Nucleotide Binding in Septin-Septin Interactions and Septin Localization in Saccharomyces cerevisiae

机译：核苷酸结合在酿酒酵母中Septin-Septin相互作用和Septin定位中的作用

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摘要

Septins are a conserved family of eukaryotic GTP-binding, filament-forming proteins. In Saccharomyces cerevisiae, five septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p, and Shs1p) form a complex and colocalize to the incipient bud site and as a collar of filaments at the neck of budded cells. Septins serve as a scaffold to localize septin-associated proteins involved in diverse processes and as a barrier to diffusion of membrane-associated proteins. Little is known about the role of nucleotide binding in septin function. Here, we show that Cdc3p, Cdc10p, Cdc11p, and Cdc12p all bind GTP and that P-loop and G4 motif mutations affect nucleotide binding and result in temperature-sensitive defects in septin localization and function. Two-hybrid, in vitro, and in vivo analyses show that for all four septins nucleotide binding is important in septin-septin interactions and complex formation. In the absence of complete complexes, septins do not localize to the cortex, suggesting septin localization factors interact only with complete complexes. When both complete and partial complexes are present, septins localize to the cortex but do not form a collar, perhaps because of an inability to form filaments. We find no evidence that nucleotide binding is specifically involved in the interaction of septins with septin-associated proteins.
机译：Septins是一个保守的真核GTP结合丝形成蛋白家族。在酿酒酵母中，五个隔膜（Cdc3p，Cdc10p，Cdc11p，Cdc12p和Shs1p）形成复合物并共定位于初生芽部位，并作为芽状细胞颈上的细丝状衣领。 Septins作为支架定位与多种过程有关的Septin相关蛋白，并作为膜相关蛋白扩散的障碍。核苷酸结合在Septin功能中的作用知之甚少。在这里，我们显示Cdc3p，Cdc10p，Cdc11p和Cdc12p均结合GTP，而P环和G4基序突变会影响核苷酸结合并导致Septin定位和功能中的温度敏感缺陷。两次杂交，体外和体内分析表明，对于所有四个septins，核苷酸结合在septin-septin相互作用和复合物形成中都很重要。在没有完整复合物的情况下，septin不会定位于皮质，这表明septin定位因子仅与完整复合物相互作用。当完全和部分复合物同时存在时，可能是因为无法形成细丝，所以隔膜位于皮质，但不形成颈圈。我们没有证据表明核苷酸结合是专门与septin相关蛋白与septins相互作用的。

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