Binding of Drosophila Orc Proteins to Anaphase Chromosomes Requires Cessation of Mitotic Cyclin-Dependent Kinase Activity

Tina Baldinger; Manfred Gossen

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Binding of Drosophila Orc Proteins to Anaphase Chromosomes Requires Cessation of Mitotic Cyclin-Dependent Kinase Activity

机译：果蝇Orc蛋白与后期染色体的结合需要停止有丝分裂细胞周期蛋白依赖性激酶活性。

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The initial step in the acquisition of replication competence by eukaryotic chromosomes is the binding of the multisubunit origin recognition complex, ORC. We describe a transgenic Drosophila model which enables dynamic imaging of a green fluorescent protein (GFP)-tagged Drosophila melanogaster ORC subunit, DmOrc2-GFP. It is functional in genetic complementation, expressed at physiological levels, and participates quantitatively in complex formation. This fusion protein is therefore able to depict both the holocomplex DmOrc1-6 and the core complex DmOrc2-6 formed by the Drosophila initiator proteins. Its localization can be monitored in vivo along the cell cycle and development. DmOrc2-GFP is not detected on metaphase chromosomes but binds rapidly to anaphase chromatin in Drosophila embryos. Expression of either stable cyclin A, B, or B3 prevents this reassociation, suggesting that cessation of mitotic cyclin-dependent kinase activity is essential for binding of the DmOrc proteins to chromosomes.

机译：真核染色体获得复制能力的第一步是多亚基起源识别复合物ORC的结合。我们描述了一个转基因的果蝇模型，该模型能够对绿色荧光蛋白（GFP）标签的 Drosophila melanogaster ORC亚基DmOrc2-GFP进行动态成像。它在遗传互补中起作用，在生理水平上表达，并定量参与复合物的形成。因此，该融合蛋白能够描绘由果蝇（Drosophila）起始蛋白形成的完整复合体DmOrc1-6和核心复合体DmOrc2-6。可以沿细胞周期和发育体内监测其定位。 DmOrc2-GFP在中期染色体上未检测到，但可与果蝇胚胎中的后期染色质快速结合。稳定的细胞周期蛋白A，B或B3的表达可阻止这种重新结合，这表明有丝分裂细胞周期蛋白依赖性激酶活性的终止对于DmOrc蛋白与染色体的结合至关重要。 展开▼

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《Molecular and Cellular Biology》 |2009年第1期|共10页

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Tina Baldinger; Manfred Gossen;
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中图分类细胞生物学;

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1. A Rho-binding protein kinase C-like activity is required for the function of protein kinase N in Drosophila development [J] . Betson M, Settleman J Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2007,第4期

机译：果蝇发育中蛋白激酶N的功能需要Rho结合蛋白激酶C样活性。

2. A Rho-Binding Protein Kinase C-Like Activity Is Required for the Function of Protein Kinase N in Drosophila Development [J] . Jeffrey Settleman, Martha Betson Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2007,第4期

机译：Rho结合蛋白激酶C类似活性是果蝇发育中蛋白激酶N的功能所必需的。

3. P38 Mitogen-activated Protein Kinase Activity Is Required during Mitosis for Timely Satisfaction of the Mitotic Checkpoint But Not for the Fidelity of Chromosome Segregation [J] . Kyunghee Lee, Alison E. Kenny, Conly L. Rieder Molecular biology of the cell . 2010,第13期

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4. Drosophila A-kinase anchor protein 200, protein kinase A and diethylether anesthesia [C] . Akihiro Tamai, Yoshiharu Tanaka, Sumiko Gam International Conference on Basic and Systemic Mechanisms of Anesthesia . 2005

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5. Exploring mechanisms that control the activity of cyclin-dependent kinase 1 during mitotic transitions in somatic cells. [D] . Potapova, Tamara. 2009

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6. Binding of Drosophila Orc Proteins to Anaphase Chromosomes Requires Cessation of Mitotic Cyclin-Dependent Kinase Activity [O] . Tina Baldinger, Manfred Gossen 2009

机译：果蝇Orc蛋白与后期染色体的结合需要停止有丝分裂细胞周期蛋白依赖性激酶活性。

7. Binding of Drosophila Orc Proteins to Anaphase Chromosomes Requires Cessation of Mitotic Cyclin-Dependent Kinase Activity ▿ † [O] . Baldinger, Tina, Gossen, Manfred 2008

机译：果蝇Orc蛋白与后期染色体的结合需要停止有丝分裂细胞周期蛋白依赖性激酶活性▿†

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Binding of Drosophila Orc Proteins to Anaphase Chromosomes Requires Cessation of Mitotic Cyclin-Dependent Kinase Activity

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