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首页> 外文期刊>Molecular and Cellular Biology >RSC Facilitates Rad59-Dependent Homologous Recombination between Sister Chromatids by Promoting Cohesin Loading at DNA Double-Strand Breaks
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RSC Facilitates Rad59-Dependent Homologous Recombination between Sister Chromatids by Promoting Cohesin Loading at DNA Double-Strand Breaks

机译：RSC通过促进DNA双链断裂处的黏着素上样来促进姐妹染色单体之间的Rad59依赖同源重组。

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Homologous recombination repairs DNA double-strand breaks by searching for, invading, and copying information from a homologous template, typically the homologous chromosome or sister chromatid. Tight wrapping of DNA around histone octamers, however, impedes access of repair proteins to DNA damage. To facilitate DNA repair, modifications of histones and energy-dependent remodeling of chromatin are required, but the precise mechanisms by which chromatin modification and remodeling enzymes contribute to homologous DNA repair are unknown. Here we have systematically assessed the role of budding yeast RSC (remodel structure of chromatin), an abundant, ATP-dependent chromatin-remodeling complex, in the cellular response to spontaneous and induced DNA damage. RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination. Multiple recombination assays revealed that RSC is uniquely required for recombination between sister chromatids by virtue of its ability to recruit cohesin at DNA breaks and thereby promoting sister chromatid cohesion. This study provides molecular insights into how chromatin remodeling contributes to DNA repair and maintenance of chromatin fidelity in the face of DNA damage.
机译：同源重组通过从同源模板（通常是同源染色体或姊妹染色单体）中搜索，侵入和复制信息来修复DNA双链断裂。然而，DNA紧密包裹在组蛋白八聚体周围，阻碍了修复蛋白对DNA的损伤。为了促进DNA修复，需要组蛋白的修饰和染色质的能量依赖性重塑，但是染色质修饰和重塑酶促进同源DNA修复的确切机制尚不清楚。在这里，我们已经系统地评估了发芽的酵母RSC（染色质的重塑结构）（一种丰富的，ATP依赖的染色质重塑复合物）在细胞对自发和诱导的DNA损伤的反应中的作用。 RSC与重组蛋白Rad59物理相互作用，并在同源重组中起作用。多种重组测定表明，RSC是姐妹染色单体之间重组的唯一必需手段，这是因为它具有在DNA断裂处募集粘着素从而促进姐妹染色单体凝聚的能力。这项研究为面对DNA损伤的染色质重塑如何促进DNA修复和保持染色质保真度提供了分子见解。

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