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首页> 外文期刊>Molecular and Cellular Biology >Recruitment of Cdc20 to the Kinetochore Requires BubR1 but Not Mad2 in Drosophila melanogaster
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Recruitment of Cdc20 to the Kinetochore Requires BubR1 but Not Mad2 in Drosophila melanogaster

机译：果蝇Cdc20的招募需要BubR1，但不需要Mad2。

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To prevent aneuploidy, cells require a mitotic surveillance mechanism, the spindle assembly checkpoint (SAC). The SAC prevents metaphase/anaphase transition by blocking the ubiquitylation and destruction of cyclin B and securin via the Cdc20-activated anaphase-promoting complex or cyclosome (APC/C)-mediated proteolysis pathway. This checkpoint involves the kinetochore proteins Mad2, BubR1, and Cdc20. Mad2 and BubR1 are inhibitors of the APC/C, but Cdc20 is an activator. Exactly how the SAC regulates Cdc20 via unattached kinetochores remains unclear; in vertebrates, most current models suggest that kinetochore-bound Mad2 is required for initial binding to Cdc20 to form a stable complex that includes BubR1. Here, we show that the Mad2 kinetochore dimerization recruitment mechanism is conserved and that the recruitment of Cdc20 to kinetochores in Drosophila requires BubR1 but not Mad2. BubR1 and Mad2 can bind to Cdc20 independently, and the interactions are enhanced after cells are arrested at mitosis by the depletion of Cdc27 using RNA interference (RNAi) in S2 cells or by MG132 treatment in syncytial embryos. These findings offer an explanation of why BubR1 is more important than Mad2 for SAC function in flies. These findings could lead to a better understanding of vertebrate SAC mechanisms.
机译：为了防止非整倍性，细胞需要有丝分裂监视机制，即纺锤体装配检查点（SAC）。 SAC通过Cdc20激活的后期促进复合物或环体（APC / C）介导的蛋白水解途径来阻断细胞周期蛋白B和丝氨酸蛋白的泛素化和破坏，从而阻止了中期/后期过渡。该检查点涉及线粒体蛋白Mad2，BubR1和Cdc20。 Mad2和BubR1是APC / C的抑制剂，而Cdc20是激活剂。尚不清楚SAC如何通过独立的动臂调节器调节Cdc20。在脊椎动物中，大多数当前模型表明，与线粒体结合的Mad2是与Cdc20初始结合以形成包括BubR1的稳定复合物所必需的。在这里，我们证明了Mad2线粒体二聚化募集机制是保守的，而将Cdc20募集到 Drosophila 中的动植物上需要BubR1，但不需要Mad2。 BubR1和Mad2可以独立地与Cdc20结合，在S2细胞中使用RNA干扰（RNAi）消耗Cdc27或通过合胞胚胎中的MG132处理，细胞在有丝分裂中被捕获后，相互作用被增强。这些发现解释了为什么BubR1对于果蝇的SAC功能比Mad2更重要。这些发现可能会导致对脊椎动物SAC机制的更好理解。

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