• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A Specific Set of Exon Junction Complex Subunits Is Required for the Nuclear Retention of Unspliced RNAs in Caenorhabditis elegans
【24h】

A Specific Set of Exon Junction Complex Subunits Is Required for the Nuclear Retention of Unspliced RNAs in Caenorhabditis elegans

机译：秀丽隐杆线虫中未剪接的RNA的核保留需要特定的外显子连接复杂亚基的集合。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The exon junction complex (EJC) is highly conserved in many organisms and is involved in various steps of mRNA metabolism. During the course of investigating the role of EJC in the germ line sex determination of the nematode Caenorhabditis elegans, we found that depletion of one of the three core subunits (Y14, MAG-1, and eukaryotic translation initiation factor 4III [eIF4AIII]) or one auxiliary subunit (UAP56) of EJC resulted in the cytoplasmic leakage of unspliced RNAs from almost all of the C. elegans protein-coding genes examined thus far. This leakage was also observed with the depletion of several splicing factors, including SF3b, IBP160, and PRP19, all of which genetically interacted with Y14. We also found that Y14 physically interacts with both pre-mRNA and spliceosomal U snRNAs, especially U2 snRNA, and that the interaction was abolished when both IBP160 and PRP19 were depleted. Our results strongly suggest that a specific set of EJC subunits is recruited onto introns and interacts with components of the spliceosome, including U2 snRNP, to provide a critical signal for the surveillance and nuclear retention of unspliced RNAs in C. elegans.
机译：外显子连接复合物（EJC）在许多生物中高度保守，并参与mRNA代谢的各个步骤。在研究EJC在确定线虫秀丽线虫的生殖系性别中的作用的过程中，我们发现三个核心亚基（Y14，MAG-1和真核翻译起始因子4III [eIF4AIII]）之一的耗竭或EJC的一个辅助亚基（UAP56）导致迄今为止尚未检测到的几乎所有秀丽隐杆线虫蛋白质编码基因的未剪接RNA发生胞质泄漏。还观察到了这种泄漏，并且耗尽了一些剪接因子，包括SF3b，IBP160和PRP19，所有这些因子均与Y14发生了遗传相互作用。我们还发现，Y14与pre-mRNA和剪接的U snRNA，特别是U2 snRNA发生物理相互作用，并且当IBP160和PRP19都耗尽时，这种相互作用被取消。我们的结果强烈表明，一组特定的EJC亚基被募集到内含子上，并与剪接体的组件（包括U2 snRNP）相互作用，从而为线虫的未剪接RNA的监视和核保留提供了关键信号。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号