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首页> 外文期刊>Molecular and Cellular Biology >The Stationary-Phase Cells of Saccharomyces cerevisiae Display Dynamic Actin Filaments Required for Processes Extending Chronological Life Span
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The Stationary-Phase Cells of Saccharomyces cerevisiae Display Dynamic Actin Filaments Required for Processes Extending Chronological Life Span

机译：酿酒酵母的固定相细胞显示动态肌动蛋白丝所需的过程，以延长时间跨度。

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Stationary-growth-phase Saccharomyces cerevisiae yeast cultures consist of nondividing cells that undergo chronological aging. For their successful survival, the turnover of proteins and organelles, ensured by autophagy and the activation of mitochondria, is performed. Some of these processes are engaged in by the actin cytoskeleton. In S. cerevisiae stationary-phase cells, F actin has been shown to form static aggregates named actin bodies, subsequently cited to be markers of quiescence. Our in vivo analyses revealed that stationary-phase cultures contain cells with dynamic actin filaments, besides the cells with static actin bodies. The cells with dynamic actin displayed active endocytosis and autophagy and well-developed mitochondrial networks. Even more, stationary-phase cell cultures grown under calorie restriction predominantly contained cells with actin cables, confirming that the presence of actin cables is linked to successful adaptation to stationary phase. Cells with actin bodies were inactive in endocytosis and autophagy and displayed aberrations in mitochondrial networks. Notably, cells of the respiratory activity-deficient cox4Δ strain displayed the same mitochondrial aberrations and actin bodies only. Additionally, our results indicate that mitochondrial dysfunction precedes the formation of actin bodies and the appearance of actin bodies corresponds to decreased cell fitness. We conclude that the F-actin status reflects the extent of damage that arises from exponential growth.
机译：固定生长阶段酿酒酵母酵母培养物由经历时间老化的非分裂细胞组成。对于它们的成功存活，进行蛋白质和细胞器的周转，这是通过自噬和线粒体的激活来确保的。肌动蛋白的细胞骨架参与其中的某些过程。在酿酒酵母固定相细胞中，F肌动蛋白已显示形成称为肌动蛋白体的静态聚集体，随后被称为静止标记。我们的体内分析表明，固定相培养物中除了具有静态肌动蛋白体的细胞外，还包含具有动态肌动蛋白丝的细胞。具有动态肌动蛋白的细胞表现出主动的内吞作用和自噬，并且线粒体网络发达。甚至更多，在热量限制下生长的固定相细胞培养物主要包含带有肌动蛋白电缆的细胞，证实了肌动蛋白电缆的存在与成功适应固定相有关。具有肌动蛋白小体的细胞在胞吞作用和自噬中没有活性，并在线粒体网络中显示出畸变。值得注意的是，呼吸活动不足的 cox4 Δ菌株的细胞仅显示相同的线粒体畸变和肌动蛋白体。此外，我们的结果表明线粒体功能障碍先于肌动蛋白体的形成，而肌动蛋白体的出现与细胞适应性降低相对应。我们得出结论，F-肌动蛋白状态反映了指数增长引起的损害程度。

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