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首页> 外文期刊>Molecular and Cellular Biology >Signaling Events of the Rim101 Pathway Occur at the Plasma Membrane in a Ubiquitination-Dependent Manner
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Signaling Events of the Rim101 Pathway Occur at the Plasma Membrane in a Ubiquitination-Dependent Manner

机译：Rim101通路的信号事件发生在泛素依赖性的血浆膜上。

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In yeast, external alkalization and alteration in plasma membrane lipid asymmetry are sensed by the Rim101 pathway. It is currently under debate whether the signal elicited by external alkalization is transduced to downstream molecules at the plasma membrane or via endocytosis of the Rim21 sensor protein at the late endosome. We found that the downstream molecules, including arrestin-related protein Rim8, calpain-like protein Rim13, and scaffold protein Rim20, accumulated at the plasma membrane upon external alkalization and that the accumulation was dependent on Rim21. Snf7, an endosomal sorting complex required for transport (ESCRT) III subunit also essential for the Rim101 pathway, localized to the plasma membrane, in addition to the late endosome, under alkaline conditions. Snf7 at the plasma membrane but not at the late endosome was shown to be involved in Rim101 signaling. In addition, the Rim101 pathway was normally activated, even when endocytosis was severely impaired. Considering this information as a whole, we propose that Rim101 signaling proceeds at the plasma membrane. We also found that activity of the Rsp5 ubiquitin ligase was required for recruiting the downstream molecules to the plasma membrane, suggesting that ubiquitination mediates Rim101 signaling at the plasma membrane.
机译：在酵母中，Rim101途径检测到外部碱化和质膜脂质不对称性的改变。目前正在争论是由外部碱化引发的信号被转导至质膜的下游分子还是通过晚期内体的Rim21传感器蛋白的内吞作用。我们发现下游分子，包括抑制蛋白相关蛋白Rim8，钙蛋白酶样蛋白Rim13和支架蛋白Rim20，在外部碱化时聚集在质膜上，并且该积累取决于Rim21。 Snf7是运输必需的内体分选复合物（ESCRT）III亚基，也是晚期Rim101途径必不可少的，在碱性条件下，Rim101途径除了后期的内体外，还位于质膜上。已显示质膜处的Snf7而非晚期内体的Snf7参与Rim101信号传导。另外，即使内吞作用严重受损，Rim101途径也通常被激活。考虑到整个信息，我们建议Rim101信号在质膜上进行。我们还发现Rsp5泛素连接酶的活性是将下游分子募集到质膜所必需的，这表明泛素化介导质膜的Rim101信号传导。

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