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首页> 外文期刊>Molecular and Cellular Biology >Utp14 Recruits and Activates the RNA Helicase Dhr1 To Undock U3 snoRNA from the Preribosome
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Utp14 Recruits and Activates the RNA Helicase Dhr1 To Undock U3 snoRNA from the Preribosome

机译：Utp14招募并激活RNA解旋酶Dhr1以使U3 snoRNA脱离原核小体

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摘要

In eukaryotic ribosome biogenesis, U3 snoRNA base pairs with the pre-rRNA to promote its processing. However, U3 must be removed to allow folding of the central pseudoknot, a key feature of the small subunit. Previously, we showed that the DEAH/RHA RNA helicase Dhr1 dislodges U3 from the pre-rRNA. DHR1 can be linked to UTP14, encoding an essential protein of the preribosome, through genetic interactions with the rRNA methyltransferase Bud23. Here, we report that Utp14 regulates Dhr1. Mutations within a discrete region of Utp14 reduced interaction with Dhr1 that correlated with reduced function of Utp14. These mutants accumulated Dhr1 and U3 in a pre-40S particle, mimicking a helicase-inactive Dhr1 mutant. This similarity in the phenotypes led us to propose that Utp14 activates Dhr1. Indeed, Utp14 formed a complex with Dhr1 and stimulated its unwinding activity in vitro. Moreover, the utp14 mutants that mimicked a catalytically inactive dhr1 mutant in vivo showed reduced stimulation of unwinding activity in vitro. Dhr1 binding to the preribosome was substantially reduced only when both Utp14 and Bud23 were depleted. Thus, Utp14 is bifunctional; together with Bud23, it is needed for stable interaction of Dhr1 with the preribosome, and Utp14 activates Dhr1 to dislodge U3.
机译：在真核生物核糖体生物发生中，U3 snoRNA碱基与前rRNA配对以促进其加工。但是，必须取下U3，以折叠中心假结，这是小亚基的关键特征。以前，我们显示DEAH / RHA RNA解旋酶Dhr1从pre-rRNA移走了U3。通过与rRNA甲基转移酶Bud23的遗传相互作用，可以将 DHR1 连接到 UTP14 ，后者编码前核糖体的必需蛋白。在这里，我们报告Utp14调节Dhr1。 Utp14离散区域内的突变减少了与Dhr1的相互作用，而Dhr1与Utp14的功能降低相关。这些突变体将Dhr1和U3积累在40S之前的颗粒中，模仿了解旋酶失活的Dhr1突变体。在表型上的相似性使我们提出Utp14激活Dhr1。实际上，Utp14与Dhr1形成了复合物，并在体外刺激了它们的放松活性。此外，模仿体内无催化活性的 dhr1 突变体 的 utp14 突变体在体外对释放放松活性的刺激减弱。 。仅当Utp14和Bud23均被耗尽时，Dhr1与原核糖体的结合才大大降低。因此，Utp14是双功能的。与Bud23一起，需要Dhr1与原核糖体稳定相互作用，而Utp14激活Dhr1使其脱离U3。

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