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首页> 外文期刊>Molecules >Effects of Refined Xiaoyaosan on Depressive-Like Behaviors in Rats with Chronic Unpredictable Mild Stress through Neurosteroids, Their Synthesis and Metabolic Enzymes
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Effects of Refined Xiaoyaosan on Depressive-Like Behaviors in Rats with Chronic Unpredictable Mild Stress through Neurosteroids, Their Synthesis and Metabolic Enzymes

机译：精制逍遥散对慢性不可预知轻度应激大鼠神经类固醇的抑郁样行为，合成及代谢酶的影响

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Abstrac t : To observe the effects of refined Xiaoyaosan (XYS) on the depressive-like behaviors in rats with chronic unpredictable mild stress (CUMS), and to explore the relationship between the changes of neurosteroids and mRNA expressions of their synthesis and metabolic enzymes, and the mechanism of XYS in the treatment of depression. Methods : Eighty-four healthy male Sprague-Dawley rats were randomly divided into normal group, model group, XYS group and fluoxetine group. The latter three groups were subjected to 21 days of CUMS to prepare the stress depression model. Rats in the XYS group, and fluoxetine group were given intragastric administration with refined XYS and fluoxetine, respectively. The behavioral changes of the rats were observed after 21 days. The contents of pregnenolone (PREG), progesterone (PROG) and alloprognanolone (ALLO) in the plasma of rats were measured by ELISA. The levels of PREG, PROG and ALLO in the hippocampus and amygdala tissues were measured by LC-MS/MS. The mRNA expressions of 3α-hydroxysteroid dehydrogenase (3α-HSD), 3β-hydroxysteroid dehydrogenase (3β-HSD), cholesterol side-chain cleavage enzyme (P450scc) and 5α-reductase (5a-R) in the hippocampus and amygdala were detected by RT-qPCR methods. Results : There were changes in the model rats. The contents of PREG, PROG and ALLO changed similarly, which reflected in the decrease of PROG and ALLO, and the increase of PREG. The mRNA expression of P450scc was increased, and the mRNA expressions of 3α-HSD, 3β-HSD and 5a-R were decreased. Refined XYS could improve the behaviors of rats and the biological indicators. Conclusion s : There is a neurosteroid dysfunction in the brain region of depression rat model animals, and the mechanism of refined XYS depression treatment may be related to the regulation of the control of mRNA expression of related synthesis and metabolic enzymes in the hippocampus and amygdala, further affecting the contents of neurosteroids.
机译：摘要：观察精制逍遥散（XYS）对慢性不可预测轻度应激（CUMS）大鼠抑郁样行为的影响，并探讨神经甾体的变化及其合成和代谢酶的mRNA表达之间的关系，和XYS治疗抑郁症的机制。方法：将84只健康雄性Sprague-Dawley大鼠随机分为正常组，模型组，XYS组和氟西汀组。后三组接受CUMS治疗21天，以建立应激抑郁模型。 XYS组和氟西汀组分别在大鼠胃内给予精制的XYS和氟西汀。 21天后观察大鼠的行为变化。用ELISA法测定大鼠血浆中孕烯醇酮（PREG），孕酮（PROG）和去甲孕烷酮（ALLO）的含量。通过LC-MS / MS测量海马和杏仁核组织中PREG，PROG和ALLO的水平。通过检测海马和杏仁核中3α-羟基类固醇脱氢酶（3α-HSD），3β-羟基类固醇脱氢酶（3β-HSD），胆固醇侧链裂解酶（P450scc）和5α-还原酶（5a-R）的mRNA表达。 RT-qPCR方法。结果：模型大鼠有变化。 PREG，PROG和ALLO的含量变化类似，反映了PROG和ALLO的减少以及PREG的增加。 P450scc的mRNA表达升高，而3α-HSD，3β-HSD和5a-R的mRNA表达降低。精制的XYS可以改善大鼠的行为和生物学指标。结论：抑郁症模型动物脑区域存在神经类固醇功能障碍，精细的XYS抑郁症治疗机制可能与调控海马和杏仁核相关合成和代谢酶的mRNA表达调控有关，进一步影响神经甾体的含量。

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