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首页> 外文期刊>Nucleic acids research >C-terminal domain (CTD) of RNA-polymerase II and N-terminal segment of the human TATA binding protein (TBP) can mediate remote and proximal transcriptional activation, respectively
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C-terminal domain (CTD) of RNA-polymerase II and N-terminal segment of the human TATA binding protein (TBP) can mediate remote and proximal transcriptional activation, respectively

机译：RNA聚合酶II的C末端结构域（CTD）和人TATA结合蛋白（TBP）的N末端区段可以分别介导远程和近端转录激活

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摘要

Activation domains of mammalian transcription factors can be subdivided into at least two functional classes. One, exemplified by the glutamine-rich activation domains of Oct and Sp1 factors, mediates transcriptional activation only from a proximal promoter position, and in response to an enhancer. The other, exemplified by the 'acidic' domain of the viral activator VP16, has the ability to activate from remote enhancer as well as from proximal promoter positions. Here we report that two proteins of the basal transcription apparatus also contain activation domains whose stimulatory effect can be detected in fusion proteins containing the GAL4 DNA binding domain. The human TATA-binding protein (TBP) contains at its N-terminus a domain with typical 'promoter' activity. We propose that the TBP N-terminal region acts as an auxiliary activation domain which reinforces the activity of other promoter-bound factors. The largest subunit of RNA polymerase II contains at its C-terminus a conserved heptad repeat structure (CTD). Both natural and synthetic CTD consensus repeats fused to GAL4 can activate transcription from remote positions like a typical enhancer-active domain. Accordingly we propose that the RNA polymerase II large subunit contains a 'portable' domain for transcriptional activation which may synergize with the activation domains of enhancer-bound transcription factors.
机译：哺乳动物转录因子的激活结构域可以细分为至少两个功能类别。以Oct和Sp1因子的富含谷氨酰胺的激活结构域为例，它仅介导近端启动子位置的转录激活，并响应增强子。另一个以病毒激活剂VP16的“酸性”结构域为例，具有从远程增强子以及近端启动子位置激活的能力。在这里我们报道基础转录装置的两个蛋白质也包含激活域，其刺激作用可以在含有GAL4 DNA结合域的融合蛋白中检测到。人TATA结合蛋白（TBP）在其N端含有一个具有典型“启动子”活性的结构域。我们建议TBP N末端区域充当辅助激活域，可增强其他启动子结合因子的活性。 RNA聚合酶II的最大亚基在其C端包含一个保守的七肽重复结构（CTD）。与GAL4融合的天然和合成CTD共有重复序列都可以激活来自远端位置的转录，例如典型的增强子活性结构域。因此，我们建议RNA聚合酶II大亚基包含一个“便携式”结构域进行转录激活，该结构域可与增强子结合的转录因子的激活结构域协同作用。

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