• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic acids research >A subtelomeric DNA sequence is required for correct processing of the macronuclear DNA sequences during macronuclear development in the hypotrichous ciliate Stylonychia lemnae
【24h】

A subtelomeric DNA sequence is required for correct processing of the macronuclear DNA sequences during macronuclear development in the hypotrichous ciliate Stylonychia lemnae

机译：亚毛端DNA序列是在毛细毛纤毛Stylonychia lemnae的大核发育过程中正确处理大核DNA序列所必需的

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During macronuclear differentiation in ciliated protozoa a series of programed DNA reorganization processes occur. These include the elimination of micronuclear-specific DNA sequences, the specific fragmentation of the genome into small gene-sized DNA molecules, the de novo addition of telomeric sequences to these DNA molecules and the specific amplification of the remaining DNA molecules. Recently we constructed a vector containing the modified micronuclear version of macronuclear destined DNA sequences that was correctly fragmented and telomeres were added de novo after injection into the developing macronucleus. It therefore must contain all the c/s-acting sequences required for these processes. We made a series of vectors deleting different sequences from the original vector. It could be shown that at least in the case studied here no micronuclear-specific sequences are required for specific fragmentation of the genome and telomere addition. However, a short subtelomeric sequence at the 3′-end is essential for these processes, whereas no specific cut seems to occur at the 5′-end. In addition, we can show that the processing activity is restricted to a short period of time during macronuclear differentiation and that a preceding transcription is required for correct processing of macronuclear-destined DNA sequences. Possible mechanisms of these processes will be discussed.
机译：在纤毛原生动物的大核分化过程中，发生了一系列程序化的DNA重组过程。这些措施包括消除微核特异性DNA序列，将基因组特异性片段化为小基因大小的DNA分子，将端粒序列从头添加到这些DNA分子以及其余DNA分子的特异性扩增。最近，我们构建了一个载体，该载体包含正确片段化的大核目的地DNA序列的修饰微核版本，端粒在注入发育中的大核后从头添加。因此，它必须包含这些过程所需的所有c / s作用序列。我们制作了一系列向量，从原始向量中删除了不同的序列。可以证明，至少在这里研究的情况下，基因组的特异性片段化和端粒添加不需要微核特异性序列。但是，对于这些过程，在3'端短的亚端粒序列是必不可少的，而在5'端似乎没有发生特异性切割。此外，我们可以证明在大核分化过程中加工活性被限制在很短的时间内，并且正确转录大核目的DNA序列需要事先进行转录。将讨论这些过程的可能机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号