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首页> 外文期刊>Nucleic acids research >Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression
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Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression

机译：拟南芥中的转录衔接子和组蛋白乙酰转移酶蛋白及其与CBF1的相互作用，CBF1是参与冷调节基因表达的转录激活因子

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The Arabidopsis CBF transcriptional activators bind to the CRT/DRE regulatory element present in the promoters of many cold-regulated genes and stimulate their transcription. Expression of the CBF1 proteins in yeast activates reporter genes carrying a minimal promoter with the CRT/DRE as an upstream regulatory element. Here we report that this ability of CBF1 is dependent upon the activities of three key components of the yeast Ada and SAGA complexes, namely the histone acetyltransferase (HAT) Gcn5 and the transcriptional adaptor proteins Ada2 and Ada3. This result suggested that CBF1 might function through the action of similar complexes in Arabidopsis. In support of this hypothesis we found that Arabidopsis has a homolog of the GCN5 gene and two homologs of ADA2, the first report of multiple ADA2 genes in an organism. The Arabidopsis GCN5 protein has intrinsic HAT activity and can physically interact in vitro with both the Arabidopsis ADA2a and ADA2b proteins. In addition, the CBF1 transcriptional activator can interact with the Arabidopsis GCN5 and ADA2 proteins. We conclude that Arabidopsis encodes HAT-containing adaptor complexes that are related to the Ada and SAGA complexes of yeast and propose that the CBF1 transcriptional activator functions through the action of one or more of these complexes.
机译：拟南芥CBF转录激活因子与许多冷调控基因启动子中存在的CRT / DRE调控元件结合，并刺激其转录。酵母中CBF1蛋白的表达激活带有CRT / DRE作为上游调控元件的最小启动子的报告基因。在这里我们报告说，CBF1的这种能力取决于酵母Ada和SAGA复合物的三个关键成分的活动，即组蛋白乙酰转移酶（HAT）Gcn5和转录衔接蛋白Ada2和Ada3。该结果表明CBF1可能通过拟南芥中类似复合物的作用起作用。为了支持该假设，我们发现拟南芥具有GCN5基因的同源物和ADA2的两个同源物，这是生物体中多个ADA2基因的首次报道。拟南芥GCN5蛋白具有固有的HAT活性，并且可以与拟南芥ADA2a和ADA2b蛋白在体外发生物理相互作用。此外，CBF1转录激活因子可以与拟南芥GCN5和ADA2蛋白相互作用。我们得出的结论是，拟南芥编码与酵母的Ada和SAGA复合物相关的含HAT衔接子复合物，并提出CBF1转录激活因子通过这些复合物中一种或多种的作用发挥功能。

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