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首页> 外文期刊>FEBS Letters >Ferredoxin‐dependent methane formation from acetate in cell extracts of Methanosarcina barkeri (strain MS)
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Ferredoxin‐dependent methane formation from acetate in cell extracts of Methanosarcina barkeri (strain MS)

机译：铁甲烷甲烷八叠球菌（MS菌株）细胞提取物中乙酸盐形成的铁氧还蛋白依赖性甲烷

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>Cell extracts of Methanosarcina barkeri grown on acetate catalyzed the conversion of acetyl-CoA to CO2 and CH4 at a specific rate of 50 nmol·min−1·mg−1. When ferredoxin was removed from the extracts by DEAE-Sephacel anion exchange chromatography, the extracts were inactive but full activity was restored upon addition of purified ferredoxin from M. barkeri or from Clostridiwn pasteurianum. The apparent Km for ferredoxin from M. barkeri was determined to be 2.5 μM. A ferredoxin dependence was also found for the formation of CO2, H2 and methylcoenzyme M from acetyl-CoA, when methane formation was inhibited by bromoethanesulfonate. Reduction of methyl-coenzyme M with H2 did not require ferredoxin. These and other data indicate that ferredoxin is involved as electron carrier in methanogenesis from acetate. Methanogenesis from acetyl-CoA in cell extracts was not dependent on the membrane fraction, which contains the cytochromes.
机译：在乙酸盐上生长的巴氏甲烷八叠球菌的细胞提取物催化乙酰辅酶A以特定的速率转化为CO 2 和CH 4 。 50 nmol·min -1 ·mg -1 。当通过DEAE-Sephacel阴离子交换色谱法从提取物中除去铁氧还蛋白时，提取物是无活性的，但是通过加入来自 M的纯化的铁氧还蛋白恢复了全部活性。 barkeri 或 Clostridiwn pasturianum 。来自 M的铁氧还蛋白的表观K m 。测得的巴克力为2.5μM。当溴乙烷磺酸盐抑制甲烷生成时，还发现铁氧还蛋白依赖性从乙酰-CoA形成CO 2 ，H 2 和甲基辅酶M。用H 2 还原甲基辅酶M不需要铁氧还蛋白。这些和其他数据表明，铁氧还蛋白作为乙酸盐的甲烷生成中的电子载体。细胞提取物中乙酰辅酶A的产甲烷作用不依赖于含有细胞色素的膜部分。

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