...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEBS Letters >Electron microscopic study of compaction of individual DNA molecules with histone H1 in surface films
【24h】

Electron microscopic study of compaction of individual DNA molecules with histone H1 in surface films

机译：电子显微镜研究表面膜中组蛋白H1压实单个DNA分子的过程

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

>Interaction of individual spread DNA molecules with histone H1 was studied by electron microscopy using the protein-free spreading technique. It was shown that in the presence of 0.2–5 μg/ml of histone H1 in hypophase there are three types of structures in electron-microscopic preparations: fibres of non-compacted DNA, compact fibres with twisted strands of duplex DNA and compacted rod-like and circular structures where the separate fibres of duplex DNA could not be distinguished. The study of compact structure morphology allows us to conclude that they are formed by side-by-side association of DNA fibres. At an increase of ionic strength there is a tendency for transition from the second to the third type structures. The latter can be explained by transition from non-cooperative to cooperative binding of histone H1 to DNA. Regularities established for interaction between DNA and histone H1 can be useful for understanding the role of histone H1 in the higher order structure organization of chromatin.
机译：使用无蛋白铺展技术，通过电子显微镜研究了单个铺展的DNA分子与组蛋白H1的相互作用。结果表明，在亚相中存在0.2-5μg/ ml的组蛋白H1时，电镜制备中存在三种类型的结构：非紧密DNA的纤维，具有双链DNA扭曲链的紧密纤维和紧密杆状结构。类和环状结构，无法区分双链DNA的单独纤维。对致密结构形态的研究使我们可以得出结论，它们是由DNA纤维的并排结合形成的。随着离子强度的增加，存在从第二类型结构过渡到第三类型结构的趋势。后者可以通过组蛋白H1从DNA的非合作结合过渡到合作结合来解释。为DNA和组蛋白H1之间的相互作用建立的规则性对于理解组蛋白H1在染色质的高级结构组织中的作用可能是有用的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号