...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>FEBS Letters >Isolation and characterisation of oxygen evolving thylakoids from the marine prokaryote Prochloron didemni
【24h】

Isolation and characterisation of oxygen evolving thylakoids from the marine prokaryote Prochloron didemni

机译：海洋原核生物Prochlorn didemni中析氧类囊体的分离与表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

>The present study describes the first successful attempt to isolate oxygen evolving thylakoids and thylakoid fragments from the marine prokaryote Prochloron didemni, a member of the recently discovered group of prochlorophytes. Oxygen evolving thylakoid membranes and fragments were isolated from seawater suspended cells of Prochloron didemni by passage of the cells through a Yeda press and subsequent differential centrifugation of the broken material. Three fractions were collected at 1000×g, 5000×g, and 30 000×g and identified by light microscopy as cells (and their fragments), thylakoids and membrane fragments, respectively. Pigment content, oxygen evolution rate and 77 K fluorescence spectra of these fractions were virtually identical. This finding indicates that the membrane fragments obtained are not enriched in photosystem II. The P680+ reduction kinetics of thylakoid membrane fragments were determined by monitoring flash induced absorption changes at 830 nm and analysing the time course of their decay. The multiphasic relaxation kinetics and their modification by NH2OH were found to be similar to those observed in cyanobacteria and plants. These findings provide an independent line of evidence for the idea of a high conservation of the basic structural and functional pattern of the water oxidising complex in all organisms that perform oxygenic photosynthesis.
机译：>本研究描述了从海洋原核生物（Prochloron didemni）中分离出放氧类囊体和类囊体片段的首次成功尝试。通过使细菌通过Yeda压榨，然后对分离的物质进行不同的离心分离，从海水中悬浮的拟南芥（Prochloron didemni）的细胞中分离出进化出类囊体的膜和碎片。分别以1000× g ，5000× g 和30 000× g 收集三部分，并通过光学显微镜鉴定为细胞（及其片段） ），类囊体和膜碎片。这些级分的颜料含量，氧气逸出速率和77 K荧光光谱实际上是相同的。该发现表明所获得的膜片段未富集光系统II。通过监测在830 nm处的闪光诱发的吸收变化并分析其衰减的时间过程，确定类囊体膜片段的P680 + 还原动力学。发现多相弛豫动力学及其被NH 2 OH修饰的现象与在蓝细菌和植物中观察到的相似。这些发现为在所有进行氧光合作用的生物体中高度保守水氧化复合物的基本结构和功能模式提供了独立的证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号