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首页> 外文期刊>FEBS Letters >Biochemical and phylogenetic analyses of methionyl‐tRNA synthetase isolated from a pathogenic microorganism, Mycobacterium tuberculosis
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Biochemical and phylogenetic analyses of methionyl‐tRNA synthetase isolated from a pathogenic microorganism, Mycobacterium tuberculosis

机译：从病原微生物结核分枝杆菌分离的甲硫氨酰tRNA合成酶的生化和系统发育分析

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> Mycobacterium tuberculosis methionyl-tRNA synthetase (MetRS) has been cloned and characterized. The protein contains class I signature sequences but lacks the Zn2+ binding motif and the C-terminal dimerization appendix that are found in MetRSs from several organisms including E. coli MetRS. Consistent with these features, the enzyme behaved as a monomer in a gel filtration chromatography and did not contain the bound Zn2+. Nonetheless, it was active to the tRNAMet of E. coli as determined by in vivo genetic complementation and in vitro reaction. Phylogenetic analysis separated the M. tuberculosis and E. coli MetRSs into prokaryote and eukaryote-archaea group, respectively. This result is consistent with the taxonomic locations of the organism but is an interesting contrast to the case of its paralogous protein, isoleucyl-tRNA synthetase, and suggests that the two enzymes evolved in separate idiosyncratic pathways.
机译：克隆并鉴定了> 结核分枝杆菌甲硫氨酰-tRNA合成酶（MetRS）。该蛋白含有I类签名序列，但缺少Zn 2 + 结合基序和C端二聚化附录，这些蛋白在包括 E在内的多种生物的MetRS中都发现。大肠杆菌 MetRS。与这些特征一致，该酶在凝胶过滤色谱中表现为单体，并且不包含结合的Zn 2 + 。但是，它对 E的tRNA Met 具有活性。体内遗传互补和体外反应测定大肠杆菌。系统发育分析分离了 M。结核病和 E。大肠埃希菌MetRSs分别分为原核生物和真核生物古细菌群。该结果与生物体的分类学位置一致，但与它的旁系蛋白异亮氨酰-tRNA合成酶的情况形成了有趣的对比，并表明这两种酶在不同的特异途径中进化。

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