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Salinity effect on production of PHA and EPS by Haloferax mediterranei

机译：盐度对 Haloferax mediterranei 的PHA和EPS产生的影响

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The halophilic archaeon, Haloferax mediterranei, is able to produce polyhydroxyalkanoates (PHAs) in large quantities. Along with the synthesis of PHAs by H. mediterranei, an extracellular polymeric substance (EPS) is excreted as a byproduct, lowering the efficiency of the production of PHAs. In this experiment, salinity effects on the carbon distribution to synthesis of PHAs and/or EPS were explored in order to control their production. It was found that high NaCl concentrations inhibited the productivity of EPS while encouraging the productivity of PHAs. The optimal salinity for the growth and proliferation of H. mediterranei was in the range of 150–200 g L?1. EPS productivity decreased from 371.36 to 319.74 mg EPS per g CDW as the concentration of NaCl increased from 75 g L?1 to 250 g L?1. However, high salinity promoted the synthesis of PHAs. When the NaCl concentration was 250 g L?1, the intracellular content of PHAs reached a maximum of 71.1%. This result indicated that a high NaCl concentration significantly stimulated the production of PHAs while depressing the production of EPS. This study provided a possible solution to adjust the carbon distribution to the synthesis of PHAs and EPS by H. mediterranei by controlling the concentration of NaCl.
机译：嗜盐古菌 Haloferax mediterranei 能够大量生产聚羟基链烷酸酯（PHA）。连同 H合成的PHA。 mediterranei 是一种细胞外聚合物（EPS）的副产物，会降低其生产效率。在该实验中，研究了盐度对PHA和/或EPS合成中碳分布的影响，以控制其生产。发现高NaCl浓度抑制了EPS的生产率，同时促进了PHA的生产率。为 H生长和增殖的最佳盐度。 mediterranei 的范围是150–200 g L ？1 。随着NaCl浓度从75 g L ？1 增至250 g L ？1，EPS生产率从每g CDW 371.36降至319.74 mg EPS。 。然而，高盐度促进了PHA的合成。当NaCl浓度为250 g L ？1 时，PHA的细胞内含量最高为71.1％。该结果表明高NaCl浓度显着刺激了PHA的产生，同时抑制了EPS的产生。这项研究提供了一种可能的解决方案，以通过 H调节碳分布以合成PHAs和EPS。通过控制NaCl的浓度

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