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首页> 外文期刊>RSC Advances >Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids
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Enantioselectivity of D-amino acid oxidase in the presence of ionic liquids

机译：离子液体存在下D-氨基酸氧化酶的对映选择性

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In this paper, enantioselectivities of D-amino acid oxidase (DAAO) in ten ionic liquids were investigated in detail. Among the ionic liquids studied, 3-methylimidazolium formate ([MIM][COO]) was able to trigger enzyme activation with L-Ala as substrate. The enzyme activity of DAAO reached a maximum of 4.26 U mg?1 min?1 in the presence of 40% [MIM][COO], while no activity toward L-Ala was observed when DAAO was incubated with other ILs. Moreover, DAAO activities towards other L-amino acids such as L-Pro and L-Arg were also improved in [MIM][COO], which indicated that the enzymatic enantioselectivity of amino acids was lowered. This was further confirmed by capillary electrophoresis, circular dichrosim and fluorescence analysis. Molecular optimization supported that the protein–ionic liquid interactions modulated the structure of the enzyme, especially in [MIM][COO], leading to relaxation in the enantioselectivity of DAAO. This observation and exploration might open a new window for modulation enantioselectivity resolution in ionic liquids.
机译：本文研究了 D -氨基酸氧化酶（DAAO）在十种离子液体中的对映选择性。在所研究的离子液体中，甲酸3-甲基咪唑鎓（[MIM] [COO]）能够以 L -Ala为底物触发酶活化。在40的存在下，DAAO的酶活最大为4.26 U mg ？1 min ？1 ％[MIM] [COO]，而DAAO与其他ILs孵育时未观察到对 L -Ala的活性。此外，在[MIM] [COO]中，DAAO对其他 L -氨基酸如 L -Pro和 L -Arg的活性也得到了改善。 ，表明氨基酸的酶对映选择性降低。毛细管电泳，圆二色谱和荧光分析进一步证实了这一点。分子优化支持蛋白质-离子液体相互作用调节了酶的结构，尤其是在[MIM] [COO]中，导致DAAO的对映选择性放松。这种观察和探索可能为离子液体中调制对映选择性的解析开辟新的窗口。

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