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Comprehensive investigation of the enzymatic oligomerization of esculin by laccase in ethanol?:?water mixtures

机译：漆酶在乙醇​​？：？水混合物中对七叶苷酶促低聚反应的综合研究

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The enzymatic polymerization of phenolic compounds arouses increasing interest due to the production of derivatives with improved biological activity. The reaction yield, the molecular mass, the structure and the properties of synthesized polymers can be controlled by the reaction conditions such as solvent and type of enzyme and substrate. In this study, the oxidative oligomerization of esculin by laccase from Trametes versicolor was performed in the presence of ethanol, a biocompatible co-solvent for food and nutraceutical applications. The formation of a precipitate was associated with the oligomerization reaction except for the medium with 50% (v/v) ethanol, due to the low reaction yield. The evaluation of antioxidant activity of the monomer and products showed that the pellet fraction from the reaction with esculin at 2 g L?1 in acetate buffer led to the highest activities. The presence of esculin oligomers was confirmed by MALDI-TOF analysis, which identified a repetition unit of 338 Da with a degree of polymerization up to 9 as well as other oligomers, mainly in the pellet fraction, with a repetition unit of 176 Da which are attributed to be esculetin oligomers. Additionally, size exclusion chromatography (SEC) and Fourier transform infrared spectroscopy (FT-IR) were used to characterize the products.
机译：由于产生具有改善的生物活性的衍生物，酚类化合物的酶促聚合引起了越来越多的关注。合成聚合物的反应收率，分子量，结构和性质可以通过反应条件如溶剂，酶和底物的类型来控制。在这项研究中，七叶树茶中漆酶对七叶树素的氧化低聚反应是在乙醇存在下进行的，乙醇是一种生物相容性食品和营养保健品的共溶剂。除了具有50％（v / v）乙醇的介质之外，由于低的反应产率，沉淀的形成与低聚反应有关。单体和产物的抗氧化活性评估表明，在乙酸缓冲液中2 g L ？1 与七叶红素反应产生的沉淀级分具有最高活性。七叶树素低聚物的存在已通过MALDI-TOF分析确认，该分析确定了聚合度高达9的338 Da的重复单元以及主要存在于颗粒级分中的其他低聚物，其中176 Da的重复单元为归因于七叶红素低聚物。此外，使用尺寸排阻色谱（SEC）和傅立叶变换红外光谱（FT-IR）表征产品。

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