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首页> 外文期刊>RSC Advances >Binding behaviors and structural characteristics of ternary complexes of β-lactoglobulin, curcumin, and fatty acids
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Binding behaviors and structural characteristics of ternary complexes of β-lactoglobulin, curcumin, and fatty acids

机译：β-乳球蛋白，姜黄素和脂肪酸三元复合物的结合行为和结构特征

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In this study, we evaluated the binding behaviors and structural characteristics of multi-ligand complex of β-lactoglobulin (β-lg) with curcumin and fatty acids. Methods of fluorescence spectroscopy, circular dichroism spectroscopy, dynamic light scattering, Turbiscan stability analysis, HPLC quantification, molecular docking studies, and molecular dynamics simulation were used. The results of fluorescence measurements indicated that β-lg was able to interact with curcumin and fatty acids at the same time, and the presence of fatty acids enhanced the binding constant of curcumin. The formation of ternary complexes with curcumin and fatty acids did not change the secondary structure of β-lg, while this process resulted in a considerable increase in particle size and negative charges compared to the native β-lg (ending up at around 145 nm and ?30 mV in the ternary complexes). The loading efficiencies of the fatty acids were 32 ± 2% in β-lg-myristic acid (C14:0)-curcumin and 34 ± 3% in β-lg-palmitic acid (C16:0)-curcumin, and those of curcumin were 58 ± 2% in β-lg-C14:0-curcumin and 57 ± 2% in β-lg-C16:0-curcumin. These findings provide basic information to clarify the binding mechanism for the interactions of β-lg with ligands at multiple sites on the molecular level.
机译：在这项研究中，我们评估了β-乳球蛋白（β-lg）与姜黄素和脂肪酸的多配体复合物的结合行为和结构特征。使用了荧光光谱，圆二色光谱，动态光散射，Turbiscan稳定性分析，HPLC定量，分子对接研究和分子动力学模拟的方法。荧光测量的结果表明，β-Ig能够同时与姜黄素和脂肪酸相互作用，并且脂肪酸的存在增强了姜黄素的结合常数。与姜黄素和脂肪酸形成的三元复合物不会改变β-Ig的二级结构，而与天然β-Ig相比，此过程会导致粒径和负电荷的显着增加（终止于145 nm和三元配合物中的？30 mV）。脂肪酸在β-lg-肉豆蔻酸（C14：0）-姜黄素中的负载效率为32±2％，在β-lg-棕榈酸（C16：0）-姜黄素中的负载效率为姜黄素β-Ig-C14：0-姜黄素为58±2％，β-Ig-C16：0-姜黄素为57±2％。这些发现为阐明β-Ig与分子水平上多个位点上的配体相互作用的结合机理提供了基本信息。

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