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首页> 外文期刊>RSC Advances >Time-dependent responses of earthworms to soil contaminated with low levels of lead as detected using 1H NMR metabolomics
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Time-dependent responses of earthworms to soil contaminated with low levels of lead as detected using 1H NMR metabolomics

机译：用 1 H NMR代谢组学检测worm对低铅污染土壤的时间依赖性响应

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1 H NMR-based metabolomics was used to profile the time-dependent metabolic responses of earthworms (Eisenia fetida) that were exposed to low-Pb-contaminated-soil (L-Pb-CS) for 28 days using an indoor culture. Earthworms were gathered after days 1, 7, 14, 21 and 28 of the exposure. The earthworm extracts were then analyzed using 500 MHz nuclear magnetic resonance. Spectrum interpretation, statistical analysis and plotting were performed using the “R” software. The metabolic trajectories, histopathological examination, Pb accumulation, survival rate and mean weights of the earthworms were evaluated to investigate and explain the possible mechanism of action. The results showed that the Pb concentrations of earthworms in the Pb exposure groups increased with longer exposure times. Pb treated groups showed varying degrees of histopathological damage. The metabolic response trajectories in the Pb treated groups were of a similar type but differed in the magnitudes of metabolite responses, which suggested that the responses of the Pb1 and Pb2 groups may involve the same metabolic mechanism. Earthworms may produce a toxic response on days 1–14, whereas a detoxification strategy was initiated during days 14–28 to adapt to the L-Pb-CS, as indicated by the negative maximum distance of metabolic trajectories. Specifically, the most serious damage in the histopathology examination and the most extreme values of metabolites were observed on the 14th day. The metabolic changes involved the amino acid, membrane, and energy metabolism of earthworms. Myo-inositol, 2-hexyl-5-ethyl-3-furansulfonate (HEFS), scyllo-inositol, succinate, alanine and maltose were found to be potential biomarkers for exposure to the L-Pb-CS. This study demonstrated that 1H NMR-based metabolomics along with metal accumulation and histological detection provide a reliable approach for interpreting time-dependent metabolic mechanisms in earthworms.
机译： 1 基于H NMR的代谢组学用于分析暴露于低Pb的earth（ Eisenia fetida ）的时间依赖性代谢反应使用室内培养的污染土壤（L-Pb-CS），持续28天。在暴露的第1、7、14、21和28天后收集gathered。然后使用500 MHz核磁共振分析worm提取物。使用“ R”软件进行光谱解释，统计分析和绘图。对evaluated的代谢轨迹，组织病理学检查，Pb积累，存活率和平均体重进行了评估，以研究和解释其可能的作用机理。结果表明，铅暴露组中worm的铅浓度随着暴露时间的延长而增加。铅治疗组表现出不同程度的组织病理学损害。 Pb处理组的代谢反应轨迹是相似的类型，但代谢物反应的幅度不同，这表明Pb1和Pb2组的反应可能涉及相同的代谢机制。 metabolic可能在第14到14天产生毒性反应，而排毒策略则在第14到28天开始适应L-Pb-CS，这由代谢轨迹的最大负距离表示。具体而言，在第14 日观察到了组织病理学检查中最严重的损伤和代谢产物的最高值。代谢变化涉及worm的氨基酸，膜和能量代谢。发现肌醇，2-己基-5-乙基-3-呋喃磺酸盐（HEFS），硬脂基肌醇，琥珀酸酯，丙氨酸和麦芽糖是暴露于L-Pb-CS的潜在生物标志物。这项研究表明， 1 基于H NMR的代谢组学以及金属积累和组织学检测为解释earth中时间依赖性代谢机制提供了可靠的方法。

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