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Label-free and sensitive detection of microalgae protein using GNRs-based resonance light scattering system

机译：使用基于GNRs的共振光散射系统无标签灵敏地检测微藻蛋白

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A novel and label-free gold nanorods (GNRs)-based resonance light scattering system assay has been developed on the basis of that the interactions between microalgae proteins and GNRs could emit strong fluorescence signal. We have prepared GNRs which were well dispersed in the solution and the microalgae protein was absorbed onto the surface of GNRs. The results demonstrated that the intensity of fluorescence has correlation with the protein concentration. The optimum pH was 5.5 and the optimum concentration of inorganic salt ions Na+ was 0.5 mol L?1; the stable time of the reaction system was 2 min. Because of the protein molecules are firmly combined with the surface of the gold particles, a protein layer is formed to prevent the aggregation of gold nanoparticles. Gold nanoparticles have a strong adsorption to proteins and other biological macromolecules and will not change their biological activity, hence it provides a number of advantages. This method offers the advantages of higher sensitivity and selectivity in microalgae protein detection and providing great potential for biology diagnosis.
机译：基于微藻蛋白与GNRs之间的相互作用可以发出强烈的荧光信号，在此基础上开发了一种新型的无标记金纳米棒（GNRs）共振光散射系统。我们制备了GNRs，它们很好地分散在溶液中，微藻蛋白被吸收到了GNRs的表面上。结果表明，荧光强度与蛋白质浓度相关。最佳pH值为5.5，无机盐离子Na + 的最佳浓度为0.5 mol L ？1 ;反应体系的稳定时间为2分钟。因为蛋白质分子与金颗粒的表面牢固结合，所以形成蛋白质层以防止金纳米颗粒的聚集。金纳米颗粒对蛋白质和其他生物大分子具有很强的吸附力，并且不会改变其生物活性，因此具有许多优势。该方法在微藻蛋白检测中具有更高的灵敏度和选择性，并为生物学诊断提供了巨大的潜力。

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