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首页> 外文期刊>RSC Advances >Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress
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Detoxifying effects of ultrafiltration fractions of Dendrobium aphyllum peptides on chemical and AAPH-induced oxidative stress

机译：铁皮石D肽超滤级分对化学和AAPH诱导的氧化应激的解毒作用

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Antioxidative peptides were extracted from Dendrobium aphyllum. The Lactobacillus strain was identified as Lactobacillus amylolyticus L6. Analysis of amino acid composition and HPLC-MS/MS analysis indicated a high antioxidative potential of the low molecular weight DA-P fractions which contained 21 different types of polypeptides. DA-P exhibited antioxidative abilities and the DA-P fraction with a size less than 1 kDa displayed optimal activity, as determined through DPPH and ABTS free radical scavenging assays and the reducing power assay. Human erythrocytes subjected to an 2,2′-azobis[2-methylpropionamidine] dihydrochloride (AAPH)-induced hemolysis assay using the DA-P fraction with the size below 1 kDa quenched the intracellular antioxidant system by inhibiting the generation of reactive oxygen species and malondialdehyde and regulating the activity of cellular antioxidative enzymes, including superoxide dismutase, catalase, and glutathione peroxidase. These results provide valuable information for the use of Dendrobium aphyllum as a functional food for Lactobacillus sp.
机译：从石D中提取抗氧化肽。该乳杆菌菌株被鉴定为解淀粉乳杆菌 L6。氨基酸组成分析和HPLC-MS / MS分析表明，低分子量DA-P馏分具有21种不同类型多肽的高抗氧化潜力。通过DPPH和ABTS自由基清除试验和还原能力试验确定，DA-P表现出抗氧化能力，大小小于1 kDa的DA-P部分表现出最佳活性。使用大小小于1 kDa的DA-P级分进行2,2'-偶氮二[2-甲基丙酰胺am]二盐酸盐（AAPH）诱导的溶血测定的人类红细胞通过抑制活性氧的产生而淬灭了细胞内的抗氧化剂系统，丙二醛和调节细胞抗氧化酶的活性，包括超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶。这些结果为使用石end 作为乳酸杆菌 sp的功能食品提供了有价值的信息。

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