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首页> 外文期刊>RSC Advances >Immobilization of copper ions on chitosan/cellulose acetate blend hollow fiber membrane for protein adsorption
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Immobilization of copper ions on chitosan/cellulose acetate blend hollow fiber membrane for protein adsorption

机译：壳聚糖/乙酸纤维素共混中空纤维膜上铜离子的固定化以吸附蛋白质

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摘要

An affinity membrane was prepared by immobilizing copper ions (Cu2+) on chitosan (CS) and cellulose acetate (CA) blend hollow fibers, and the affinity membrane (denoted as CS/CA-Cu) was evaluated for its performance in the adsorption of a model protein, Bovine Serum Albumin (BSA). The analysis indicated that the amount of copper ions coupled on CS/CA-Cu reached around 3.9 mg per g-membrane. The experimental results showed that CS/CA-Cu achieved a high adsorption capacity of the 69 mg-BSA per g-membrane, under the typical solution condition of pH at 7.4 and ionic strength at 0.12 M, with the nonspecific adsorption being as low as less than 15%. The adsorption isotherm followed the Langmuir model and the adsorption kinetics exhibited both transport-limited and attachment-limited behaviors. CS/CA-Cu also performed favorably in the neutral pH range and at low ionic strength.
机译：通过将铜离子（Cu 2 + ）固定在壳聚糖（CS）和醋酸纤维素（CA）混合中空纤维上制备亲和膜，并将亲和膜（表示为评估CS / CA-Cu）在吸附模型蛋白牛血清白蛋白（BSA）中的性能。分析表明，与CS / CA-Cu偶联的铜离子量达到每克膜3.9 mg。实验结果表明，在典型的pH值为7.4和离子强度为0.12 M的典型溶液条件下，CS / CA-Cu达到了每克膜69 mg-BSA的高吸附容量，非特异性吸附低至少于15％。吸附等温线遵循Langmuir模型，并且吸附动力学表现出运输受限和附着受限行为。 CS / CA-Cu在中性pH范围和低离子强度下也表现出色。

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