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首页> 外文期刊>RSC Advances >Beneficial effects of Trametes versicolor pretreatment on saccharification and lignin enrichment of organosolv-pretreated pinewood
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Beneficial effects of Trametes versicolor pretreatment on saccharification and lignin enrichment of organosolv-pretreated pinewood

机译：云芝预处理对有机溶剂预处理松木糖化和木质素富集的有益作用

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While previous studies have shown that white-rot fungal pretreatment reduces the severity of chemical pretreatments and improves enzymatic saccharification yields, very few have investigated the synergistic effects of fungal pretreatment combined with organosolv pretreatment on lignin recovery and quality. In this study, loblolly pine chips were incubated with Trametes versicolor for 15, 30 and 45 days, prior to organosolv pretreatment and enzymatic saccharification. Fungal pretreatment for 15 days improved the saccharification yield by 23%, and higher amounts (56%) of lignin-enriched fractions were obtained. Fungal pretreatment for 45 days led to extensive depolymerization, structural modification and enrichment of lignin in the organosolv precipitates (OP). Characterization of the OP fraction showed that samples pretreated for 30 and 45 days contained higher amounts of hydroxyl groups and p-hydroxyphenyl (H) subunits, and showed increases in depolymerization of carbohydrates as well as decreases in G/H lignin ratio. It became apparent that further investigations are needed to explore the benefits of combining fungal and organosolv pretreatments for improving lignin yields from softwoods.
机译：尽管先前的研究表明，白腐真菌预处理可降低化学预处理的严重程度并提高酶促糖化产率，但很少有人研究了真菌预处理与有机溶剂预处理联合使用对木质素回收和品质的协同作用。在这项研究中，在有机溶剂预处理和酶促糖化之前，将火松木片与 Trametes versicolor 孵育15、30和45天。进行15天的真菌预处理后，糖化率提高了23％，并获得了更多（56％）的木质素富集级分。真菌预处理45天导致了有机溶剂沉淀（OP）中木质素的广泛解聚，结构修饰和富集。 OP馏分的表征表明，经过30天和45天预处理的样品含有更高数量的羟基和 p -羟苯基（H）亚基，并且碳水化合物解聚的增加以及G /的降低木质素比。显然，需要进一步研究以探索将真菌和有机溶剂预处理结合使用以提高软木木质素产量的益处。

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