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首页> 外文期刊>RSC Advances >Effects of triphala and guggul aqueous extracts on inhibition of protein fibrillation and dissolution of preformed fibrils
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Effects of triphala and guggul aqueous extracts on inhibition of protein fibrillation and dissolution of preformed fibrils

机译：雷公藤和古根胶水提物对蛋白原纤化和预成纤维溶解的抑制作用

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Herbal preparations have long been used for treatment of variety of diseases. However, a systematic and?scientific evaluation of their effects and mechanisms of action have been lacking, although it is believed that synergistic complementation of the effects of the multiple chemical components in these preparations would be playing crucial regulatory roles in the different metabolic pathways which may interfere in a biological system. In this context, we investigate here protein fibrillation which is known to be an important process responsible for many neurodegenerative and other diseases. Using lysozyme as a model protein, we have studied using a combination of spectroscopic and microscopic techniques, the effects of two herbal preparations, derived from triphala and guggul, on the fibrillation process. We observed that both the extracts have the ability to inhibit protein fibrillation but triphala has much more dominating influence on fibrillation inhibition. The kinetics of fibrillation has been monitored by fluorescence spectroscopy as well as absorption spectroscopy, and the morphological changes associated with fibrillation have been monitored by transmission electron microscopy. Our experiments seem to suggest an interference (or synergy), though minor, between the effects of triphala and guggul on the fibrillation/aggregation process. We also observed that both triphala and guggul have the ability to dissolve preformed fibrils and aggregates of lysozyme, in a synergistic manner. We believe, the present indication of possible synergy between triphala and guggul can be conceptually extrapolated to the components of the individual preparations. Thus, the current work has significant therapeutic implications and will provide scientific basis to the development of new generation of phytopharmaceuticals which can be used alone or in combination with other drugs.
机译：草药制剂长期以来一直用于治疗多种疾病。然而，尽管据信在这些制剂中多种化学成分的作用的协同补充将在不同的代谢途径中起关键的调节作用，但仍缺乏对其作用和作用机理的系统和科学评估。干扰生物系统。在这种情况下，我们在这里研究蛋白原纤化，这是导致许多神经退行性疾病和其他疾病的重要过程。我们使用溶菌酶作为模型蛋白，结合光谱学和显微镜技术研究了两种来自三叶草和古古耳的草药制剂对原纤维形成过程的影响。我们观察到，两种提取物均具有抑制蛋白原纤化的能力，但三叶草对原纤化抑制的影响更大。已通过荧光光谱法和吸收光谱法监测了原纤化的动力学，并已通过透射电子显微镜监测了与原纤化有关的形态变化。我们的实验似乎表明，Triphala和Guggul对原纤化/聚集过程的影响虽然很小，但还是有干扰（或协同作用）。我们还观察到，Triphala和Guggul均具有以协同方式溶解预制的原纤维和溶菌酶聚集体的能力。我们认为，目前关于三叶草和含漱剂之间可能存在协同作用的迹象，可以从概念上推断到各个制剂的成分中。因此，当前的工作具有重要的治疗意义，并将为新一代植物药物的开发提供科学依据，这些植物药物可以单独使用或与其他药物联合使用。

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