• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Influence of a facile pretreatment process on lipid extraction from Nannochloropsis sp. through an enzymatic hydrolysis reaction
【24h】

Influence of a facile pretreatment process on lipid extraction from Nannochloropsis sp. through an enzymatic hydrolysis reaction

机译：简便的预处理工艺对 Nannochloropsis sp。脂质提取的影响。通过酶促水解反应

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microalgal lipid is being considered as an alternative source of ω-3 polyunsaturated fatty acids (ω-3 PUFAs); however, the extraction of ω-3 PUFA-rich algal lipid still needs further research. In this study, single enzyme and their combinations were screened based on their cell wall disrupting capability and lipid recovery yield, and economical and feasible extraction parameters were determined. Lipid recovery could reach 22.18 ± 0.26 wt% when algae was treated with an 8?:?1 (w/w) algae/cocktail enzyme ratio and a 2?:?1 cellulase/lysozyme mass ratio at 50 °C under stirring for 5 h at a pH of 4.0, and the content of PUFAs was as high as 23.30%. In addition, alkaline pretreatment efficiently facilitates the degradation of algal cell wall in the enzymatic hydrolysis, and it has been verified that cell wall ruptures via two steps: swelling by a weak alkali pretreatment and decomposition by enzymatic hydrolysis. Furthermore, all the extraction operations can take place under facile conditions. These results imply that algal lipid extracted by a weak alkali pretreatment aiding enzymatic hydrolysis, is the most appropriate raw material for ω-3 PUFAs.
机译：微藻脂质被认为是ω-3多不饱和脂肪酸（ω-3PUFA）的替代来源。然而，富含ω-3PUFA的藻类脂质的提取仍需进一步研究。在这项研究中，根据其细胞壁破坏能力和脂质回收率筛选了单一酶及其组合，并确定了经济可行的提取参数。在8°C：？1（w / w）藻类/鸡尾酒酶比和2？：？1纤维素酶/溶菌酶质量比在50°C下搅拌5处理藻类时，脂质回收率可达到22.18±0.26 wt％。 pH值为4.0时，PUFAs的含量高达23.30％。另外，碱预处理有效地促进了酶水解中藻类细胞壁的降解，并且已经证实细胞壁通过两个步骤破裂：弱碱预处理引起的溶胀和酶水解引起的分解。此外，所有提取操作都可以在方便的条件下进行。这些结果表明，通过弱碱预处理提取的藻类脂质有助于酶水解，是最适合ω-3PUFAs的原料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号