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首页> 外文期刊>RSC Advances >A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe
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A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe

机译：基于sup分子探针诱导monomer探针的单体-准分子发射跃迁的乙酰胆碱酯酶活性比率荧光分析和抑制剂筛选

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摘要

A ratiometric fluorescence assay for acetylcholinesterase activity is established, which is based on controlled perylene probe assembly and monomer–excimer transition. In a buffer solution, a perylene probe with two negatively charged groups (PDI-DHA) mainly exists in monomeric form. In the presence of cationic lauroylcholine and lauric acid, PDI-DHA can form supramolecular assemblies and the perylene excimer emission can be observed. AChE can catalyze the hydrolysis of lauroylcholine to anionic lauric acid and choline. The hydrolysis process can trigger the breakdown of the supramolecular assemblies. The perylene excimer recovers to the monomeric form because of the de-aggregation of the probe. The excimer–monomer transition can be detected, and a ratiometric fluorescence assay for AChE activity and inhibitor screening is therefore established.
机译：建立了基于比例的line探针组装和单体-准分子跃迁的乙酰胆碱酯酶活性荧光定量测定法。在缓冲溶液中，带有两个带负电基团（PDI-DHA）的per探针主要以单体形式存在。在阳离子月桂酰胆碱和月桂酸的存在下，PDI-DHA可以形成超分子组装，并且可以观察到the准分子的发射。 AChE可以催化月桂酰胆碱水解为阴离子月桂酸和胆碱。水解过程可以触发超分子组装体的分解。由于探针的解聚集，exc准分子恢复为单体形式。可以检测到受激准分子向单体的转变，因此建立了用于AChE活性和抑制剂筛选的荧光定量分析法。

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