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The chemical redox modulated switch-on fluorescence of carbon dots for probing alkaline phosphatase and its application in an immunoassay

机译：用于探测碱性磷酸酶的碳点的化学氧化还原调制的接通荧光及其在免疫测定中的应用

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Early diagnosis is an important requirement for the early treatment of diseases and reliable detection methods for biomarkers are indispensable tools for early diagnosis. However, recent innovations in analytical technology are rarely employed widely due to multiple factors including the requirement of specialized equipment and infrastructures. Hence, much attention has been focused on exploring universal nanoprobes for routine practices. Here, we report a chemical redox modulated switch-on fluorescence strategy based on versatile carbon dots (CDs) for probing alkaline phosphatase (ALP) and human IgG (a model target). The fluorescence of the CDs is initially quenched by Fe(III) and then restored by ascorbic acid, which is generated from the hydrolysis of ascorbic acid 2-phosphate (AAP) by the catalysis of ALP. Based on that, a simple “switch-on” fluorescence assay for ALP was designed and leads to a sensitivity of as low as 0.8 U L?1. The proposed assay is applied in ALP sensing in human serum samples. Additionally, the application of the switch-on fluorescence strategy is successfully expanded to the immunoassay of human IgG in serum from healthy adults and patients with systemic lupus erythematosus. The developed sensing system could be devised for various target sensors and has promising potential in the field of biological analysis and clinical diagnostics.
机译：早期诊断是疾病早期治疗的重要要求，而可靠的生物标志物检测方法是早期诊断必不可少的工具。但是，由于包括专用设备和基础设施在内的多种因素，分析技术的最新创新很少被广泛采用。因此，许多注意力集中在探索通用纳米探针用于常规实践上。在这里，我们报告了基于多功能碳点（CD）的化学氧化还原调节的开启荧光策略，用于探测碱性磷酸酶（ALP）和人IgG（模型目标）。 CD的荧光首先被Fe（ III ）淬灭，然后被抗坏血酸恢复，这是抗坏血酸2-磷酸酯（AAP）通过ALP催化水解而产生的。在此基础上，设计了一种简单的“接通” ALP荧光测定法，其灵敏度低至0.8 U L ？1 。拟议的分析方法用于人类血清样品的ALP传感。此外，开关荧光策略的应用已成功地扩展到健康成年人和系统性红斑狼疮患者血清中人IgG的免疫测定。开发的传感系统可以设计用于各种目标传感器，并且在生物学分析和临床诊断领域具有广阔的发展潜力。

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