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首页> 外文期刊>RSC Advances >Determination of brain injury biomarkers by surface-enhanced Raman scattering using hollow gold nanospheres
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Determination of brain injury biomarkers by surface-enhanced Raman scattering using hollow gold nanospheres

机译：空心金纳米球表面增强拉曼散射测定脑损伤生物标志物

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The development of rapid, highly sensitive detection methods for neuron-specific enolase (NSE) and S100-β protein is very important as the levels of NSE and S100-β protein in the blood are closely related to brain injury. Therefore, we can use NSE and S100-β protein concentration detection to realize the preliminary judgment of brain injury. In this paper, we report that a simple label-free three dimensional hierarchical plasmonic nano-architecture has been designed for the sensitive surface-enhanced Raman scattering immunosensor detection of NSE and S100-β. Owing to the active group of the hollow gold nanospheres (HAuNPs), the redox molecules 4-mercaptobenzoic acid (4-MBA) and Nile blue A (NBA) absorb antibodies and provide signal generation. The prepared HAuNPs@4-MBA and HAuNPs@NBA are used as probes to easily construct a surface-enhanced Raman scattering immunosensor. When protein biomarkers are present, the sandwich nanoparticles are captured over the substrate, forming a confined plasmonic field, leading to an enhanced electromagnetic field in intensity and in space. As a result, the Raman reporter molecules are exposed to a high density of “hot spots”, which remarkably amplify the Raman signal, improving the sensitivity of the surface-enhanced Raman scattering immunosensor. Under the optimized conditions, the linear range of the proposed immunosensor is from 0.2 to 22 ng mL?1 for both NSE and S100-β. The lowest detectable concentration is 0.1 and 0.06 ng mL?1 for NSE and S100-β, respectively. The assay results for serum samples with the proposed method were in a good agreement with the standard enzyme-linked immunosorbent assay method. The proposed immunosensor is promising in clinical diagnosis. This method, which utilizes the surface-enhanced Raman scattering of HAuNPs, has great potential in the detection of biomarkers, which are vital in medical diagnoses and disease monitoring.
机译：快速，高度灵敏的神经元特异性烯醇化酶（NSE）和S100-β蛋白检测方法的开发非常重要，因为血液中NSE和S100-β蛋白的水平与脑损伤密切相关。因此，我们可以利用NSE和S100-β蛋白浓度检测来实现对脑损伤的初步判断。在本文中，我们报道了一个简单的无标记三维分层等离激元纳米体系结构已被设计用于灵敏的表面增强拉曼散射免疫传感器检测NSE和S100-β。由于空心金纳米球（HAuNPs）的活性基团，氧化还原分子4-巯基苯甲酸（4-MBA）和尼罗蓝A（NBA）吸收抗体并提供信号产生。制备的HAuNPs @ 4-MBA和HAuNPs @ NBA用作探针，可轻松构建表面增强的拉曼散射免疫传感器。当存在蛋白质生物标记物时，三明治纳米颗粒会被捕获在底物上，形成受限的等离子体场，从而导致强度和空间电磁场增强。结果，拉曼报告分子暴露于高密度的“热点”，这显着放大了拉曼信号，从而提高了表面增强型拉曼散射免疫传感器的灵敏度。在优化的条件下，对于NSE和S100-β，所提出的免疫传感器的线性范围均为0.2到22 ng mL ？1 。 NSE和S100-β的最低可检测浓度分别为0.1和0.06 ng mL ？1 。所提出的方法对血清样品的测定结果与标准的酶联免疫吸附测定法非常吻合。提出的免疫传感器在临床诊断中很有前途。该方法利用HAuNPs的表面增强拉曼散射，在检测生物标志物方面具有巨大潜力，这些标志物在医学诊断和疾病监测中至关重要。

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