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首页> 外文期刊>RSC Advances >Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp. CQ2 from Changqing oil field, China
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Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp. CQ2 from Changqing oil field, China

机译：分离和表征生物表面活性剂生产和柴油降解假单胞菌。中国长庆油田的CQ2

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In the present research investigation, 13 indigenous bacteria (from CQ1 to CQ13) were isolated from soil collected from Changqing oil field of Xi'an, China. Four promising biosurfactant producers (CQ1, CQ2, CQ4, and CQ13) were selected through primary screening among these 13 strains, including via drop collapse and oil-spreading methods. However, only the strain CQ2 showed the best biosurfactant production and was further screened by hemolytic assay, cetyl trimethyl ammonium bromide (CTAB), surface tension and emulsifying activity. The bacterium CQ2 has the ability to produce about 3.015 g L ~(?1) of biosurfactant using glucose as the sole carbon source without any optimization. The produced biosurfactant could greatly reduce surface tension from 72.66 to 24.72 mN m ~(?1) with a critical micelle concentration (CMC) of 30 mg L ~(?1) and emulsify diesel oil up to 60.1%. The cell-free broth was found to be stable in wide temperature (4–100 °C), pH (6–12) and salinity (2–20%) ranges for surface and emulsifying activity. This biosurfactant was preliminarily found to be of a glycolipid nature as evident from thin-layer chromatographic (TLC) and Fourier transform infra-red spectroscopic (FTIR) analyses. Moreover, CQ2 was able to degrade 54.7% of diesel oil, which surprisingly could form a substantial amount of bioflocculants during the degradation process. Furthermore, the 16S rDNA sequence using the Genbank BLAST tool revealed that isolated CQ2 was closely related to species of Pseudomonas genus and, thus, was entitled Pseudomonas sp. CQ2. The results of residual diesel oil contents measured by GC-MS showed that C7–C28 hydrocarbons could be degraded by Pseudomonas sp. CQ2. Thus, these findings revealed that CQ2 could be applied for remediation of diesel oil/petroleum-contaminated waters and soils on a large scale.
机译：在本研究调查中，从中国西安长庆油田采集的土壤中分离出13种本地细菌（CQ1至CQ13）。通过初步筛选从这13个菌株中选择了四个有前途的生物表面活性剂生产商（CQ1，CQ2，CQ4和CQ13），包括通过液滴崩解和涂油方法。然而，只有菌株CQ2表现出最佳的生物表面活性剂生产，并通过溶血分析，十六烷基三甲基溴化铵（CTAB），表面张力和乳化活性进一步筛选。使用葡萄糖作为唯一碳源，细菌CQ2能够产生约3.015 g L〜（？1）的生物表面活性剂，而无需进行任何优化。所生产的生物表面活性剂可将表面张力从72.66 mN m（？1）大大降低至24.72 mN m？（？1），临界胶束浓度（CMC）为30 mg L〜（？1），并乳化柴油至60.1％。发现无细胞肉汤在很宽的温度（4–100°C），pH（6–12）和盐度（2–20％）范围内均具有稳定的表面和乳化活性。从薄层色谱（TLC）和傅里叶变换红外光谱（FTIR）分析中可以明显看出，该生物表面活性剂具有糖脂性质。此外，CQ2能够降解54.7％的柴油，令人惊讶的是在降解过程中会形成大量的生物絮凝剂。此外，使用Genbank BLAST工具的16S rDNA序列显示分离出的CQ2与假单胞菌属物种密切相关，因此被命名为假单胞菌。 CQ2。 GC-MS测定残留柴油含量的结果表明，假单胞菌可降解C7–C28碳氢化合物。 CQ2。因此，这些发现表明CQ2可以大规模地用于修复被柴油/石油污染的水和土壤。

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