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The expression and biological function of the PHF2 gene in breast cancer

机译：PHF2基因在乳腺癌中的表达及其生物学功能

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PHD Finger Protein 2 (PHF2), as a protein code and a transcription regulatory gene, is a member of the Jumonji-C domain (JmjC). PHF2 is located at human chromosome 9q22.31 and is frequently decreased in various malignancies. However, the definite role of PHF2 in breast cancer remains unclear. To detect the expression and function of PHF2 in breast cancer, a q-PCR assay was used to detect the mRNA expression of PHF2 in breast cancer cell lines and paired breast cancer tissues, and immunohistochemistry was used to test the protein expression in breast cancer tissues and adjacent tissues. In addition, an adenovirus vector system was utilized to upregulate the expression of PHF2 in breast cancer cells. In our study, we found that PHF2 was down-expressed in breast cancer on both the mRNA and protein levels and the low expression of PHF2 was significantly associated with lymph node metastasis, Ki67 positive rate, ER negative expression and poor prognosis in breast cancer patients. The ectopic expression of PHF2 obviously inhibited the proliferation of breast cancer cell lines and the growth of xenograft tumors. Due to the tumor suppressor signature of PHF2 in breast cancer, we have reasons to believe that it could be a promoting marker and target for the prognosis and therapy of breast cancer.
机译：PHD Finger Protein 2（PHF2），作为蛋白质代码和转录调控基因，是Jumonji-C域（JmjC）的成员。 PHF2位于人类染色体9q22.31，在各种恶性肿瘤中经常减少。然而，尚不清楚PHF2在乳腺癌中的确切作用。为了检测PHF2在乳腺癌中的表达和功能，采用q-PCR法检测乳腺癌细胞系和配对的乳腺癌组织中PHF2的mRNA表达，并使用免疫组织化学方法检测乳腺癌组织中的蛋白表达。和邻近的组织。此外，腺病毒载体系统被用于上调乳腺癌细胞中PHF2的表达。在我们的研究中，我们发现乳腺癌中PHF2的mRNA和蛋白水平均低表达，而PHF2的低表达与乳腺癌患者的淋巴结转移，Ki67阳性率，ER阴性表达和预后不良密切相关。 。 PHF2的异位表达明显抑制了乳腺癌细胞系的增殖和异种移植肿瘤的生长。由于PHF2在乳腺癌中具有抑癌作用，因此我们有理由相信它可能是乳腺癌预后和治疗的促进性标志物和靶标。

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